| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deuel, Megan G (14)
|
NIVICT |
25.71 |
25.56 |
-0.15 |
| 2 |
Sargent, Madison L (13)
|
NIVICT |
26.25 |
26.75 |
0.50 |
| 3 |
Senglaub, Emma (13)
|
NIVICT |
27.23 |
27.17 |
-0.06 |
| 4 |
Hahn, Elizabeth P (13)
|
NISDSC |
26.80 |
27.45 |
0.65 |
| 5 |
Felix, Paige C (14)
|
NIVICT |
27.87 |
28.73 |
0.86 |
| 6 |
DeVinney, Grace E (14)
|
NIJETS |
27.81 |
28.84 |
1.03 |
| 7 |
Bromage, Charlotte C (13)
|
NIUN-Q |
28.70 |
29.18 |
0.48 |
| 8 |
Doughty, Emma J (14)
|
NIHAST |
28.00 |
29.36 |
1.36 |
| 9 |
Welsh, Gabrielle (14)
|
NIWGSC |
30.11 |
29.60 |
-0.51 |
| 10 |
Chadwick, Alina V (14)
|
NISDSC |
30.20 |
30.13 |
-0.07 |
| 11 |
Flora, Kari A (13)
|
NIJETS |
32.20 |
30.20 |
-2.00 |
| 12 |
Strom, Nina C (14)
|
NIJETS |
29.91 |
30.51 |
0.60 |
| 13 |
Wilck, Emilena R (12)
|
NINEWW |
|
30.63 |
- |
| 14 |
Kennedy, Madaliene F (14)
|
NISDSC |
31.24 |
30.69 |
-0.55 |
| 15 |
Geoca, Zoey L (14)
|
NISDSC |
30.65 |
30.94 |
0.29 |
| 16 |
Hotaling, Asha G (13)
|
NIUN-Q |
30.96 |
31.05 |
0.09 |
| 17 |
Kocher, Casey A (14)
|
NISDSC |
30.81 |
31.78 |
0.97 |
| 18 |
McFarland, Emily S (13)
|
NIVICT |
31.28 |
31.87 |
0.59 |
| 19 |
Vogt, Jael R (13)
|
NIUN-Q |
32.82 |
32.63 |
-0.19 |
| 20 |
Davison, Asella M (14)
|
NISDSC |
31.50 |
32.81 |
1.31 |
| 21 |
Thorne, Lillian E (12)
|
NIJETS |
32.73 |
33.06 |
0.33 |
| 22 |
Cafarelli, Brianna N (9)
|
NIUN-Q |
32.47 |
33.27 |
0.80 |
| 23 |
Johnson, Amber N (13)
|
NINEWW |
|
33.97 |
- |
| 24 |
Quinmore, Isabelle L (12)
|
NISDSC |
34.24 |
34.06 |
-0.18 |
| 25 |
Pierce, Marek J (10)
|
NINEWW |
|
36.72 |
- |
| 26 |
Cassidy, Alexa E (12)
|
NISDSC |
33.71 |
37.06 |
3.35 |
| 27 |
Sponable, Julia E (13)
|
NIGRAT |
48.11 |
40.20 |
-7.91 |
| 28 |
Wells, Lindsay A (10)
|
NINEWW |
|
40.84 |
- |
|
Stepanek, Livia G (14)
|
NIHAST |
29.30 |
NS |
- |