| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murillo, Jorge (28)
|
NCTAC |
55.98 |
54.63 |
-1.35 |
| 2 |
Ewing, Kendall J (17)
|
VATIDE |
54.96 |
59.66 |
4.70 |
| 3 |
Tomlinson, Alexander L (17)
|
NCTAC |
1:00.18 |
1:00.92 |
0.74 |
| 4 |
Martinez, Roman D (17)
|
NCTAC |
1:00.81 |
1:01.69 |
0.88 |
| 5 |
Lee, Henry T (15)
|
NCTAC |
1:00.70 |
1:02.90 |
2.20 |
| 6 |
Wang, Oliver Y (15)
|
NCTAC |
1:01.77 |
1:03.80 |
2.03 |
| 7 |
Spain, Noah J (16)
|
VATIDE |
1:00.10 |
1:03.99 |
3.89 |
| 8 |
Michel, Kaelig F (17)
|
VATIDE |
1:02.41 |
1:05.42 |
3.01 |
| 9 |
Barakey, Lucas A (15)
|
VATIDE |
1:02.13 |
1:06.03 |
3.90 |
| 10 |
Kruthoff, Paul C (15)
|
VATIDE |
1:06.02 |
1:07.14 |
1.12 |
| 11 |
Patricio Taylor, Christian R (19)
|
NCTAC |
1:05.99 |
1:07.58 |
1.59 |
| 12 |
Adley, Logan W (15)
|
NCUN-T |
1:06.50 |
1:09.19 |
2.69 |
| 13 |
Miranda, Austin M (15)
|
VATIDE |
1:08.10 |
1:09.29 |
1.19 |
| 14 |
Williams, Keith E (15)
|
NCTAC |
1:03.95 |
1:09.72 |
5.77 |
| 15 |
Southern, Mark T (16)
|
VATIDE |
1:04.93 |
1:09.83 |
4.90 |
| 16 |
Reed, John P (17)
|
VATIDE |
1:10.06 |
1:09.91 |
-0.15 |
| 17 |
Cobb, Jonathan Ryan E (15)
|
NCUN-T |
1:09.33 |
1:09.97 |
0.64 |
| 18 |
Biersack, Joseph M (15)
|
NCTAC |
1:18.80 |
1:17.79 |
-1.01 |
| 19 |
Lau, Anthony L (17)
|
VATIDE |
|
1:19.53 |
- |
| 20 |
Hopkins, Garrett P (15)
|
VATIDE |
|
1:22.32 |
- |
| 21 |
Krause, Peter L (15)
|
VATIDE |
1:46.36 |
1:26.05 |
-20.31 |
|
Teller, Alec R (15)
|
VATIDE |
1:04.93 |
NS |
- |
|
Lauchengco, Connor X (16)
|
NCTAC |
1:08.74 |
NS |
- |