| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ong, Thea (10)
|
MAUNSJ |
|
45.35 |
- |
| 2 |
Smallwood, Charlotte R (10)
|
MARKRY |
46.55 |
46.04 |
-0.51 |
| 3 |
Jacobson, Raegan P (9)
|
MAWAVE |
|
47.50 |
- |
| 4 |
Merch, Laura A (10)
|
MASJAC |
|
50.47 |
- |
| 5 |
Wagner, Kailey M (10)
|
MAMAR |
53.21 |
52.02 |
-1.19 |
| 6 |
Bobalik, Vivian R (9)
|
MAGRA |
|
53.07 |
- |
| 7 |
Jarantow, Alexandra K (9)
|
MAPENN |
|
54.96 |
- |
| 8 |
Jacob, Emily C (9)
|
MAWAVE |
|
56.75 |
- |
| 9 |
Brown, Charlotte L (10)
|
MAYBCC |
52.25 |
57.46 |
5.21 |
| 10 |
Johnson, Olivia M (9)
|
MAUNUD |
|
57.58 |
- |
| 11 |
Corrielus, Jayna A (10)
|
MASJAC |
|
58.15 |
- |
| 12 |
Schroeder, Caroline K (9)
|
MAECCC |
58.73 |
59.00 |
0.27 |
| 13 |
Montgomery, Talyah N (10)
|
MASJAC |
|
59.20 |
- |
| 14 |
Baron, Isabella M (10)
|
MAECCC |
59.58 |
59.32 |
-0.26 |
| 15 |
Xia, Yudiandian (9)
|
MAUNSJ |
|
1:02.91 |
- |
| 16 |
Bridgeford, Grace B (9)
|
MAECCC |
|
1:03.97 |
- |
| 17 |
Davis, Adelaide Q (9)
|
MAECCC |
1:18.23 |
1:12.09 |
-6.14 |
|
Ethiraj, Sanjita (9)
|
MAT85 |
|
DQ |
- |