| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rothrock, Abigale P (9)
|
MDCAA |
55.03 |
43.24 |
-11.79 |
| 2 |
Mackey, Allyson (Ally) C (9)
|
MDEST |
46.24 |
44.29 |
-1.95 |
| 3 |
Hutchins, Rachel M (9)
|
MDCAA |
1:05.76 |
44.93 |
-20.83 |
| 4 |
Svejda, Josephine M (9)
|
MDEST |
|
45.94 |
- |
| 5 |
Foster, Ava (9)
|
MDMAC |
46.26 |
48.71 |
2.45 |
| 6 |
Conley, Kennedy M (9)
|
MDFAST |
50.92 |
50.64 |
-0.28 |
| 7 |
Carpenter, Kendall N (9)
|
MDCAA |
|
53.32 |
- |
| 8 |
Silverman, Solona C (9)
|
MDMAS |
1:00.00 |
54.82 |
-5.18 |
| 9 |
Sargeant, Delaney O (9)
|
MDMAC |
1:00.19 |
55.78 |
-4.41 |
| 10 |
Baker, Avery B (9)
|
MDMAC |
54.53 |
56.32 |
1.79 |
| 11 |
Satterfield, Julie M (9)
|
MDGTAC |
53.22 |
57.10 |
3.88 |
| 12 |
Lotito, Jillian C (9)
|
MDMAC |
|
59.10 |
- |
| 13 |
Wollenweber, Olivia B (9)
|
MDEST |
1:06.21 |
59.88 |
-6.33 |
| 14 |
Callahan, Kate R (9)
|
MDMAC |
1:09.43 |
1:06.63 |
-2.80 |
| 15 |
Dawson, Mikayla A (9)
|
MDACA |
|
1:11.46 |
- |