| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Seligman, Micah C (9)
|
MDEST |
45.56 |
44.71 |
-0.85 |
| 3 |
Rhee, Ryan (9)
|
MDCAA |
53.29 |
46.45 |
-6.84 |
| 4 |
Cabbagestalk Jr., Travone J (9)
|
MDBCSC |
50.83 |
48.02 |
-2.81 |
| 5 |
Dorsey, David R (9)
|
MDCAA |
47.88 |
48.46 |
0.58 |
| 6 |
Wang, Timothy S (9)
|
MDCAA |
|
49.51 |
- |
| 9 |
Hall, Justin R (9)
|
MDCAA |
|
51.48 |
- |
| 11 |
Yu, Christopher B (9)
|
MDCAA |
55.77 |
52.43 |
-3.34 |
| 12 |
Will, Charles E (9)
|
MDEST |
|
53.14 |
- |
| 13 |
Padu-Kiganiis, Kaliin K (9)
|
MDCAA |
|
53.71 |
- |
| 14 |
Winstead, Amir J (9)
|
MDEST |
57.06 |
1:00.79 |
3.73 |
| 16 |
Bernstein, Tobias E (9)
|
MDCAA |
|
1:02.41 |
- |
| 17 |
Scott, Colin J (9)
|
MDEST |
|
1:03.28 |
- |
| 18 |
Zhang, Jincheng J (9)
|
MDCAA |
|
1:06.11 |
- |
| 19 |
Oster, Maksymilian I (9)
|
MDEST |
|
1:06.73 |
- |
| 20 |
Smith, Nathaniel E (9)
|
MDEST |
|
1:13.65 |
- |
|
Hendrickson, Matthew E (9)
|
MDEST |
1:05.28 |
DQ |
- |
|
Duncan, Dalton I (9)
|
MDCAA |
1:05.05 |
DQ |
- |
|
Devine, Jackson C (9)
|
MDFSC |
|
DQ |
- |
|
Marston, Samuel A (9)
|
MDFSC |
|
DQ |
- |