| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burnette, Hannah-Mae E (10)
|
MDFAST |
49.43 |
47.01 |
-2.42 |
| 2 |
Kim, Ashley Y (10)
|
MDCAA |
|
48.28 |
- |
| 3 |
Jonas, Lindsay G (10)
|
MDFSC |
47.83 |
49.04 |
1.21 |
| 4 |
Bell, Kaitlyn H (10)
|
MDFSC |
53.18 |
49.26 |
-3.92 |
| 5 |
McCoyd, Laura J (10)
|
MDFSC |
|
49.67 |
- |
| 6 |
Kim, Kate M (10)
|
MDCAA |
|
49.78 |
- |
| 7 |
Segura, McKenna A (10)
|
MDGTAC |
50.03 |
50.00 |
-0.03 |
| 8 |
Afolabi-Brown, Faith O (10)
|
MDCAA |
|
51.06 |
- |
| 9 |
Coleman, Emilie A (10)
|
MDCAA |
|
51.36 |
- |
| 10 |
Daly, Hannah C (10)
|
MDCAA |
55.40 |
51.72 |
-3.68 |
| 11 |
Thompson, Madeline B (10)
|
MDCAA |
53.91 |
52.63 |
-1.28 |
| 12 |
Simmons, Nicole E (10)
|
MDFSC |
55.66 |
54.57 |
-1.09 |
| 13 |
Aliftiras, Caroline R (10)
|
MDFSC |
54.74 |
55.07 |
0.33 |
| 14 |
Kefauver-Olsen, Hannah I (10)
|
MDMAS |
53.64 |
55.30 |
1.66 |
| 15 |
Subramanian, Priyanka (10)
|
MDFSC |
1:05.80 |
55.56 |
-10.24 |
| 16 |
Bucchioni, Alayna K (10)
|
MDRAC |
58.52 |
56.30 |
-2.22 |
| 17 |
Ellis, Cheyenne A (10)
|
MDMAS |
1:01.06 |
56.88 |
-4.18 |
| 18 |
Yanes, Bianca A (10)
|
MDFAST |
|
59.50 |
- |
| 19 |
Gittings, Hayden M (10)
|
MDEST |
57.65 |
59.81 |
2.16 |
| 20 |
Lu, Emma L (10)
|
MDCAA |
|
1:02.15 |
- |
| 21 |
Rodriguez, Alison (10)
|
MDJCC |
|
1:06.08 |
- |
| 22 |
Castillo, Lailah (10)
|
MDFSC |
|
1:08.20 |
- |
|
Hirschmann, Navah G (10)
|
MDJCC |
|
DQ |
- |
|
Keane, Rachel (10)
|
MDJCC |
53.13 |
NS |
- |
|
Belgi, Anoushka A (10)
|
MDMAS |
53.68 |
NS |
- |
|
Santosh, Sudharsna (10)
|
MDMAS |
|
NS |
- |