| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guo, Yubo E (9)
|
MDMAC |
38.32 |
26.81 |
-11.51 |
| 2 |
Husson, Wesley (9)
|
MDBAY |
44.21 |
27.14 |
-17.07 |
| 3 |
Delaney, Nolan (9)
|
MDBAY |
33.27 |
27.67 |
-5.60 |
| 4 |
Mulcahy, Grayson T (9)
|
MDFSC |
|
28.13 |
- |
| 4 |
Sellers, Sawyer J (9)
|
MDFSC |
40.67 |
28.13 |
-12.54 |
| 6 |
Nguyen, William M (9)
|
MDMAC |
|
28.98 |
- |
| 7 |
Susann, Jacob R (9)
|
MDFSC |
32.05 |
29.87 |
-2.18 |
| 8 |
Du, Evan M (9)
|
MDMAC |
30.82 |
30.72 |
-0.10 |
| 9 |
Liu, Yuchen (9)
|
MDEST |
34.50 |
31.47 |
-3.03 |
| 10 |
Cooper, Gavin (9)
|
MDBAY |
49.56 |
32.74 |
-16.82 |
| 11 |
Quintyne, Liam (9)
|
MDBAY |
|
32.90 |
- |
| 12 |
Coleman, Brandt P (9)
|
MDFSC |
|
33.01 |
- |
| 13 |
House, Grant I (9)
|
MDMAC |
42.84 |
33.58 |
-9.26 |
| 14 |
Shen, David (9)
|
MDMAC |
34.00 |
34.10 |
0.10 |
|
Stonesifer, Alexander (9)
|
MDBAY |
|
DQ |
- |