| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleck, Kristina S (12)
|
PVNCAP |
34.52 |
35.03 |
0.51 |
| 2 |
Padilla, Kayla (12)
|
PVDCPR |
|
40.20 |
- |
| 3 |
Barclay, Kate A (11)
|
PVDCPR |
41.43 |
40.61 |
-0.82 |
| 4 |
Smith, Aurelia J (11)
|
PVDCPR |
46.09 |
41.41 |
-4.68 |
| 5 |
Ipakchi, Ariana M (12)
|
PVNCAP |
43.89 |
42.19 |
-1.70 |
| 6 |
Brinkley-Tyson, Malaijah A (11)
|
PVDCPR |
45.01 |
43.48 |
-1.53 |
| 7 |
Delfino, Hannah M (12)
|
PVDCPR |
49.41 |
45.40 |
-4.01 |
| 8 |
Boyd, Adeline A (11)
|
PVDCPR |
53.19 |
45.90 |
-7.29 |
| 9 |
Manigo-Truell dos Sa, Do (11)
|
PVDCPR |
49.41 |
46.27 |
-3.14 |
| 10 |
Walker, Sydney L (12)
|
PVDCPR |
49.76 |
47.20 |
-2.56 |
| 11 |
Ferguson, Teagan E (11)
|
PVDCPR |
48.15 |
49.44 |
1.29 |
| 12 |
Harrus, Shirel B (12)
|
PVDCPR |
52.92 |
50.22 |
-2.70 |
| 13 |
Pijpers, Florijn J (11)
|
PVDCPR |
|
50.88 |
- |
| 14 |
Alexander-Fettus, Helen H (11)
|
PVDCPR |
55.88 |
50.93 |
-4.95 |
| 15 |
Buckman, Zoe H (11)
|
PVDCPR |
58.02 |
56.08 |
-1.94 |
| 16 |
Phillips, Jonna L (11)
|
PVDCPR |
|
59.98 |
- |
| 17 |
Forrest, Simone M (12)
|
PVDCPR |
|
1:09.33 |
- |
|
Peabodyhh, Holly O (11)
|
PVDCPR |
54.34 |
NS |
- |