| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gutierrez, Keira M (12)
|
PVNCAP |
35.25 |
33.70 |
-1.55 |
| 2 |
Holley, Palmer S (12)
|
PVSDS |
34.80 |
34.31 |
-0.49 |
| 3 |
Restrepo, Maria C (12)
|
PVNCAP |
38.17 |
38.03 |
-0.14 |
| 4 |
Kumar, Neha (12)
|
PVDCPR |
40.87 |
38.35 |
-2.52 |
| 5 |
Knight, Anna S (12)
|
PVNCAP |
37.40 |
38.71 |
1.31 |
| 6 |
Smittle, Carlin R (12)
|
PVSDS |
37.51 |
38.93 |
1.42 |
| 7 |
Orlic, Anastasia L (11)
|
PVSDS |
39.55 |
39.55 |
0.00 |
| 8 |
Marchand, Anna A (12)
|
PVSDS |
40.08 |
41.13 |
1.05 |
| 9 |
Kay, Sandra P (12)
|
PVTANK |
47.14 |
46.00 |
-1.14 |
| 10 |
Faye, Jaelyn Y (11)
|
PVNCAP |
47.70 |
46.02 |
-1.68 |
| 11 |
Smith, Dorothy G (12)
|
PVDCPR |
41.41 |
46.11 |
4.70 |
| 12 |
Ipakchi, Ariana M (11)
|
PVNCAP |
44.02 |
46.41 |
2.39 |
| 13 |
Guadagno, Gabriella Floriann (12)
|
PVDCPR |
|
48.59 |
- |
| 14 |
Padilla, Kayla (12)
|
PVDCPR |
46.00 |
49.47 |
3.47 |
| 15 |
Lasut, Tess E (11)
|
PVSDS |
|
49.88 |
- |
| 16 |
Ferguson, Teagan E (11)
|
PVDCPR |
|
53.68 |
- |
| 17 |
Williams, Jasmine T (11)
|
PVDCPR |
1:04.78 |
1:05.82 |
1.04 |
| 18 |
Manigo-Truell dos Sa, Do (11)
|
PVDCPR |
|
1:11.27 |
- |