| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Quentin G (12)
|
IADSMY |
39.09 |
39.15 |
0.06 |
| 2 |
Nguyen, David M (12)
|
IADSMY |
39.75 |
39.68 |
-0.07 |
| 3 |
Schechinger, Abe (12)
|
IANVFY |
42.05 |
41.51 |
-0.54 |
| 4 |
Comer, Ethan (12)
|
IANEWT |
40.88 |
42.68 |
1.80 |
| 5 |
Wigant, Kaegan P (12)
|
IADSMY |
44.39 |
44.09 |
-0.30 |
| 6 |
Draheim, Tanner (11)
|
IADSMY |
44.74 |
44.40 |
-0.34 |
| 7 |
Gravett, Mason (11)
|
IAOTT |
46.76 |
46.11 |
-0.65 |
| 8 |
Andersson, Anton V (12)
|
IADSMY |
|
46.17 |
- |
| 9 |
Breit, Aiden J (12)
|
IADSMY |
55.52 |
51.42 |
-4.10 |
|
Beebe, William E (11)
|
IADSMY |
52.03 |
DQ |
- |
|
Henrichsen, Parker R (11)
|
IADSMY |
|
DQ |
- |