| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Newman, Ainslee G (8)
|
VASMAC |
49.23 |
47.17 |
-2.06 |
| 2 |
Bustos, Vivianna (7)
|
VAACAC |
|
47.54 |
- |
| 3 |
Paradise, Lily C (7)
|
VAGATR |
53.37 |
48.23 |
-5.14 |
| 4 |
Luikart, Abigail R (7)
|
VAGATR |
51.54 |
48.80 |
-2.74 |
| 5 |
Prout, Parker J (8)
|
VAACAC |
1:06.85 |
49.42 |
-17.43 |
| 6 |
Zhang, Mira (8)
|
VAACAC |
|
49.96 |
- |
| 7 |
Xie, Sophia S (7)
|
VAACAC |
51.47 |
51.35 |
-0.12 |
| 8 |
Hendricksen, Anna K (6)
|
VAGATR |
|
51.67 |
- |
| 9 |
Kirtz, Nora (8)
|
VASMAC |
55.83 |
52.92 |
-2.91 |
| 10 |
Riner, Ella R (6)
|
VAGATR |
1:00.92 |
53.14 |
-7.78 |
| 11 |
Harris, Charlotte R (7)
|
VASMAC |
1:04.44 |
53.24 |
-11.20 |
| 12 |
Dotson, Hannah L (8)
|
VAACAC |
|
55.19 |
- |
| 13 |
Sun, Grace Y (7)
|
VAACAC |
|
57.04 |
- |
| 14 |
Heale, Hadlie R (6)
|
VASMAC |
1:04.81 |
57.31 |
-7.50 |
| 15 |
Chen, Sarah R (8)
|
VAACAC |
|
59.33 |
- |
| 16 |
Malloy, Tristen (6)
|
VASMAC |
1:14.02 |
59.43 |
-14.59 |
| 17 |
Xie, Zoe I (6)
|
VAACAC |
1:04.34 |
59.48 |
-4.86 |
| 18 |
Lyu, Elaine Y (6)
|
VAACAC |
|
1:02.71 |
- |
| 19 |
Ellington, Mia A (8)
|
VASMAC |
|
1:02.95 |
- |
| 20 |
Weston, Annie S (8)
|
VAACAC |
|
1:03.83 |
- |
| 21 |
Chung, Alyssa S (5)
|
VAACAC |
|
1:04.14 |
- |
| 22 |
Brown, Grace M (5)
|
VAACAC |
1:33.19 |
1:24.58 |
-8.61 |
|
Hollingsworth, Berkeley J (6)
|
VAACAC |
|
DQ |
- |