| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roche, Samuel L (16)
|
NIFAST |
25.41 |
26.16 |
0.75 |
| 2 |
Wahl, Alec J (18)
|
NIWEBS |
25.67 |
26.77 |
1.10 |
| 3 |
Wahl, Zachary J (26)
|
NIWEBS |
25.22 |
27.45 |
2.23 |
| 4 |
Williamson, Ethan L (17)
|
NIKVAC |
35.00 |
27.51 |
-7.49 |
| 5 |
LaRue, Carl V (14)
|
NIQUIK |
27.71 |
27.88 |
0.17 |
| 6 |
Roney, Dylan J (19)
|
NIFAST |
27.57 |
27.91 |
0.34 |
| 7 |
Fargo, Jacob M (14)
|
NIPACK |
30.26 |
28.73 |
-1.53 |
| 8 |
Dwyer, Logan A (14)
|
NIKVAC |
35.00 |
29.87 |
-5.13 |
| 9 |
Healy, John C (15)
|
NIWEBS |
32.60 |
29.89 |
-2.71 |
| 10 |
Russotti, Daniel J (16)
|
NIWEBS |
30.24 |
30.26 |
0.02 |
| 11 |
Anderson P C Davies, Tiago (15)
|
NIPACK |
31.74 |
30.50 |
-1.24 |
| 12 |
Joseph, Julian C (13)
|
NIPACK |
30.27 |
30.99 |
0.72 |
| 13 |
Williams, Giovanni C (12)
|
NIPACK |
32.18 |
31.21 |
-0.97 |
| 14 |
Fay, Sean D (14)
|
NIFAST |
36.19 |
31.90 |
-4.29 |
| 15 |
Hahn, Kurt C (13)
|
NISDSC |
33.14 |
31.94 |
-1.20 |
| 16 |
Greenfield, Zachary P (15)
|
NIKVAC |
35.00 |
32.09 |
-2.91 |
| 17 |
Magda, Dylan J (12)
|
NIUN-S |
32.82 |
32.60 |
-0.22 |
| 18 |
Nenadic, Alexander B (14)
|
NIFAST |
37.75 |
32.81 |
-4.94 |
| 19 |
Deutsch, Benjamin E (15)
|
NIKVAC |
38.00 |
33.10 |
-4.90 |
| 20 |
Goltsman, Joshua A (14)
|
NIPACK |
42.88 |
34.07 |
-8.81 |
|
Harris, Liam B (13)
|
NIKVAC |
40.00 |
DQ |
- |
|
Sambhus, Laksh (11)
|
NIPACK |
41.19 |
NS |
- |
|
Ott, David C (13)
|
NIFAST |
36.60 |
NS |
- |