| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LaRue, Carl V (14)
|
NIQUIK |
41.94 |
32.75 |
-9.19 |
| 2 |
Naccarella, Antonia L (17)
|
NIWEBS |
34.87 |
35.15 |
0.28 |
| 3 |
Sokolowski, Suzanne N (13)
|
NIWEBS |
37.44 |
35.46 |
-1.98 |
| 4 |
LaRue, Jessie A (16)
|
NIQUIK |
33.02 |
36.56 |
3.54 |
| 5 |
Guilfoil, Ella K (12)
|
NIFAST |
36.78 |
36.64 |
-0.14 |
| 6 |
Dinh, Nakisha V (11)
|
NIWEBS |
38.84 |
38.51 |
-0.33 |
| 7 |
Solodova, Elena A (12)
|
NIFAST |
37.98 |
38.88 |
0.90 |
| 8 |
Shen, Alivia (11)
|
NIPACK |
43.93 |
39.01 |
-4.92 |
| 9 |
Bromage, Charlotte C (13)
|
NIQUIK |
43.13 |
40.38 |
-2.75 |
| 10 |
Markham, Rory P (12)
|
NIFAST |
33.75 |
40.95 |
7.20 |
| 11 |
Corby, Owen T (11)
|
NIPACK |
41.02 |
41.25 |
0.23 |
| 12 |
Cropper, Iala K (12)
|
NIFAST |
44.01 |
44.24 |
0.23 |
| 13 |
Caldwell, Hannah M (11)
|
NIQUIK |
46.15 |
44.46 |
-1.69 |
| 14 |
Warren, Grace C (14)
|
NIWEBS |
42.07 |
47.13 |
5.06 |
| 15 |
Visconte, Adriana E (11)
|
NIFAST |
54.12 |
50.13 |
-3.99 |
| 16 |
Uchal, Charlotte J (8)
|
NIWEBS |
52.52 |
51.12 |
-1.40 |
| 17 |
Cole, Bruno M (10)
|
NIPACK |
49.27 |
51.52 |
2.25 |
| 18 |
Cougle, Adam J (11)
|
NIFAST |
57.44 |
56.55 |
-0.89 |
| 19 |
Boggio, Addison L (8)
|
NIQUIK |
1:10.00 |
1:00.23 |
-9.77 |
| 20 |
Weber, Sophia R (7)
|
NIWEBS |
1:00.00 |
1:00.48 |
0.48 |
| 21 |
Frenzel, Jack W (9)
|
NIWEBS |
54.41 |
1:00.77 |
6.36 |
| 22 |
LaDelfa, Sophia L (9)
|
NIWEBS |
54.71 |
1:03.15 |
8.44 |
| 23 |
Uchal, Lilah A (6)
|
NIWEBS |
1:07.10 |
1:06.42 |
-0.68 |
| 24 |
Nacca, Giada F (8)
|
NIFAST |
1:01.00 |
1:09.58 |
8.58 |