| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fazili, Saara (11)
|
NIPACK |
33.88 |
33.35 |
-0.53 |
| 2 |
Minnigh, Ella N (12)
|
NIPACK |
34.22 |
33.58 |
-0.64 |
| 3 |
Campbell, Brooklyn M (12)
|
NIVICT |
36.92 |
33.74 |
-3.18 |
| 4 |
Patel, Sia M (11)
|
NIPACK |
34.01 |
34.08 |
0.07 |
| 5 |
Stonehouse, Sierra L (12)
|
NIGRSC |
35.55 |
34.95 |
-0.60 |
| 6 |
Stefanou, Ava C (11)
|
NIQUIK |
35.95 |
36.28 |
0.33 |
| 7 |
Pospiech, Morgan R (12)
|
NIFAST |
38.34 |
36.30 |
-2.04 |
| 8 |
Kinslow, Erin E (11)
|
NIPACK |
37.61 |
36.31 |
-1.30 |
| 9 |
Thurston, Lauren A (11)
|
NIGCOM |
41.59 |
36.83 |
-4.76 |
| 10 |
Buck, Kathryn E (12)
|
NIWEBS |
37.29 |
37.33 |
0.04 |
| 11 |
Ozbas, Melek (11)
|
NIWEBS |
39.13 |
37.90 |
-1.23 |
| 12 |
Jones, Lillian M (12)
|
NIFAST |
|
38.99 |
- |
| 13 |
Greisch, Alisha D (11)
|
NIGRSC |
38.45 |
39.18 |
0.73 |
| 14 |
Nitchman, Melina E (11)
|
NIWEBS |
40.31 |
39.23 |
-1.08 |
| 15 |
Farina, Samantha R (11)
|
NIGRSC |
38.74 |
39.33 |
0.59 |
| 16 |
Cady, Makenna N (12)
|
NIWEBS |
44.04 |
40.11 |
-3.93 |
| 17 |
Hackett, Grace C (11)
|
NIIRON |
40.20 |
41.48 |
1.28 |
| 18 |
Cabral, Aurora (12)
|
NIRRST |
41.11 |
42.06 |
0.95 |
| 19 |
Raimondo, Erin E (12)
|
NIGRSC |
49.14 |
45.85 |
-3.29 |
| 20 |
Suplicki, Skye V (11)
|
NIHAST |
59.18 |
52.39 |
-6.79 |
|
Inzinga, Rose Marie (12)
|
NIWEBS |
37.07 |
DQ |
- |
|
Urtis, Julia M (11)
|
NIIRON |
40.01 |
NS |
- |