| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Parks, Abigail M (8)
|
OHLAKY |
36.19 |
23.12 |
-13.07 |
| 2 |
Perdue, Cherokee (8)
|
OHPCYP |
26.04 |
23.28 |
-2.76 |
| 3 |
Montgomery, Madeline E (8)
|
OHPCYP |
22.15 |
23.30 |
1.15 |
| 4 |
Hill, Elizabeth J (8)
|
OHSPY |
23.17 |
24.11 |
0.94 |
| 5 |
Wilhelm, Faith R (7)
|
OHLAKY |
|
24.94 |
- |
| 6 |
Strickle, Bailey L (7)
|
OHCFY |
|
25.30 |
- |
| 7 |
Christ, Gracianna E (8)
|
OHKEY |
|
25.45 |
- |
| 8 |
Godemann, Evelyn K (7)
|
OHKEY |
27.98 |
26.05 |
-1.93 |
| 9 |
Gandhi, Jeyanyaa (7)
|
OHUCY |
|
26.24 |
- |
| 10 |
Wang, Emily (7)
|
OHKEY |
37.03 |
27.52 |
-9.51 |
| 11 |
Kirchner, Genevieve A (8)
|
OHCFY |
|
27.80 |
- |
| 12 |
Haas, Bella E (7)
|
OHKEY |
35.87 |
29.52 |
-6.35 |
| 13 |
Brown, Emma L (7)
|
OHLAKY |
34.96 |
31.24 |
-3.72 |
| 14 |
Stuckey, Allie M (7)
|
OHSPY |
|
32.54 |
- |
| 15 |
Ackerman, Ella R (8)
|
OHLAKY |
|
32.67 |
- |
| 16 |
Morgan, Murphy E (8)
|
OHCFY |
|
35.95 |
- |
| 17 |
Shuping, Virginia R (7)
|
OHSPY |
|
38.84 |
- |
| 18 |
Gatch, Persephone A (8)
|
OHKEY |
|
39.38 |
- |
| 19 |
Derr, Olympia S (7)
|
OHSPY |
|
41.87 |
- |
|
Smith, Ashtyn J (7)
|
OHKEY |
|
DQ |
- |
|
Springhart, Kamryn Y (7)
|
OHKEY |
|
DQ |
- |
|
Beecroft, Gretchen E (7)
|
OHCFY |
23.35 |
NS |
- |