| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Westerman, Ryan C (10)
|
MAUN-1 |
46.15 |
40.47 |
-5.68 |
| 2 |
Lever, Owen P (10)
|
MACBST |
44.89 |
41.92 |
-2.97 |
| 3 |
Kim, Joshua (9)
|
MAJW |
46.46 |
44.47 |
-1.99 |
| 4 |
Schutta, Benjamin H (10)
|
MAFSS |
52.02 |
46.11 |
-5.91 |
| 5 |
Macko, Rohan M (10)
|
MAJW |
48.89 |
47.27 |
-1.62 |
| 6 |
Maiale, Aidan A (10)
|
MAPVAC |
47.03 |
48.05 |
1.02 |
| 7 |
Smolders, Colin W (9)
|
MAACST |
50.03 |
48.52 |
-1.51 |
| 8 |
Heim, Carter N (10)
|
MAACST |
54.69 |
49.28 |
-5.41 |
| 9 |
Snook, Jayden M (9)
|
MAGRA |
52.80 |
49.56 |
-3.24 |
| 10 |
Mannion, Brendan S (9)
|
MAGRA |
51.12 |
49.65 |
-1.47 |
| 11 |
Lee, Ross N (10)
|
MAFSS |
51.78 |
51.23 |
-0.55 |
| 12 |
Pileggi, Robert J (10)
|
MAGRA |
52.49 |
51.25 |
-1.24 |
| 13 |
Izhakov, Daniel (10)
|
MANSC |
1:00.00 |
51.30 |
-8.70 |
| 14 |
Line, Greyson B (10)
|
MAUN-1 |
|
51.49 |
- |
| 15 |
Pijawka, Christopher M (9)
|
MARAD |
55.00 |
52.07 |
-2.93 |
| 16 |
McCrane, Evan M (9)
|
MANSC |
1:00.00 |
54.51 |
-5.49 |
| 17 |
Citara, Chase J (9)
|
MAACST |
53.96 |
54.84 |
0.88 |
| 18 |
Jestice, Mason T (9)
|
MAACST |
59.00 |
58.47 |
-0.53 |
| 19 |
Purumandla, Abhinav R (9)
|
MAJW |
1:10.00 |
1:04.80 |
-5.20 |
| 20 |
Jawork, Jack (9)
|
MAFSS |
55.00 |
1:06.22 |
11.22 |
|
Izhakov, Gabriel (9)
|
MANSC |
53.00 |
DQ |
- |
|
Ross, Trevor P (10)
|
MACAA |
50.64 |
NS |
- |