| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Loomis, Amanda (11)
|
FGECAC |
35.87 |
37.84 |
1.97 |
| 2 |
Hayden, Avery (11)
|
FGLLL |
40.27 |
38.88 |
-1.39 |
| 3 |
Salcedo, Sara F (11)
|
FGLLL |
39.00 |
38.92 |
-0.08 |
| 4 |
Volot, Maya (12)
|
FGLLL |
41.70 |
40.44 |
-1.26 |
| 5 |
Sharp, Angelina J (12)
|
FGLLL |
39.03 |
40.74 |
1.71 |
| 6 |
Pointer, Madison A (11)
|
FGJDST |
46.66 |
40.90 |
-5.76 |
| 7 |
Cobb, Kylie M (11)
|
FGBMSC |
42.59 |
41.42 |
-1.17 |
| 8 |
Roy, Lillian K (11)
|
FGLLL |
43.05 |
41.45 |
-1.60 |
| 9 |
Williams, Sarah R (12)
|
FGWOW |
47.91 |
44.26 |
-3.65 |
| 10 |
Bishai, Angela (11)
|
FGLLL |
48.39 |
46.91 |
-1.48 |
| 11 |
Blair, Jenna A (11)
|
FGMART |
45.51 |
48.77 |
3.26 |
| 12 |
Jones, Emily R (12)
|
FGJDST |
48.59 |
49.17 |
0.58 |
| 13 |
Friedman, Rose N (11)
|
FGLLL |
|
52.04 |
- |
|
Rasmussen, Isabella M (12)
|
FGLLL |
|
NS |
- |
|
Wagner, Olivia F (11)
|
FGLLL |
50.19 |
NS |
- |