| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Katz, Sophie (10)
|
MNMSI |
40.40 |
40.28 |
-0.12 |
| 2 |
Liu, Sophia W (10)
|
OKOK |
42.82 |
42.40 |
-0.42 |
| 3 |
Strahammer, Lauryn O (10)
|
ILIL |
42.30 |
42.63 |
0.33 |
| 4 |
Mathiason, Madeline R (9)
|
MNMSI |
|
42.72 |
- |
| 5 |
Rubel, Nola A (10)
|
MWMW |
42.78 |
42.85 |
0.07 |
| 6 |
Bruns, Linnea K (10)
|
NDND |
42.99 |
43.03 |
0.04 |
| 7 |
Enenbach, Olivia C (10)
|
MWMW |
|
43.71 |
- |
| 8 |
Tangpong, Nashatawn Y (10)
|
NDND |
|
45.11 |
- |
| 9 |
Qian, Anna H (10)
|
OKOK |
|
46.10 |
- |
| 10 |
Marsh, Ella G (10)
|
MVMV |
|
48.02 |
- |
| 11 |
Lawrence-Benz, Lillian E (10)
|
ARAR |
|
49.68 |
- |
| 12 |
Penturf, Caroline A (10)
|
ARAR |
|
50.06 |
- |