| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dal Ponte, Adriana R (10)
|
ILLWSA |
43.25 |
41.25 |
-2.00 |
| 2 |
Strahammer, Lauryn O (10)
|
ILGSC |
43.64 |
42.30 |
-1.34 |
| 3 |
Manak, Helen W (9)
|
ILBRRY |
44.14 |
42.86 |
-1.28 |
| 4 |
Caicedo, Olivia M (10)
|
ILACAD |
46.87 |
44.11 |
-2.76 |
| 5 |
Patkar, Ananya (10)
|
ILCWAC |
46.59 |
44.18 |
-2.41 |
| 6 |
Walker, Drew M (10)
|
ILFMC |
46.08 |
44.36 |
-1.72 |
| 7 |
Sun, Nicole Z (10)
|
ILFMC |
44.33 |
44.56 |
0.23 |
| 8 |
Chandrakasan, Shreya (10)
|
ILPAC |
45.33 |
45.12 |
-0.21 |
| 9 |
Riekena, Bryn R (10)
|
ILDDST |
40.06 |
45.30 |
5.24 |
| 10 |
Jennings, Anna M (10)
|
ILFFSC |
46.86 |
46.07 |
-0.79 |
| 11 |
Buhrfiend, Elizabeth R (10)
|
ILFMC |
47.80 |
46.36 |
-1.44 |
| 12 |
Stephens, Piper A (10)
|
ILLWSA |
48.31 |
46.37 |
-1.94 |
| 13 |
Allegra, Mia Bella A (10)
|
ILHSC |
46.13 |
46.44 |
0.31 |
| 14 |
Piriyakulvej, Palinee P (10)
|
ILWILD |
45.25 |
46.59 |
1.34 |
| 15 |
Weisgerber, Jocelyn M (10)
|
ILWHTN |
46.83 |
47.47 |
0.64 |
| 16 |
Mulcrone, Delaney G (10)
|
ILCWAC |
48.03 |
48.12 |
0.09 |
| 17 |
Graham, Gabriella R (9)
|
ILACAD |
48.45 |
48.73 |
0.28 |
| 18 |
Niemiec, Jennifer (10)
|
ILLIFE |
48.77 |
50.04 |
1.27 |
| 19 |
Komarova, Anastasia (10)
|
ILRA |
52.26 |
50.17 |
-2.09 |