| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ladha, Natalia K (12)
|
FGSF |
|
38.83 |
- |
| 2 |
Harries, Calista D (12)
|
FGSOFL |
43.75 |
40.38 |
-3.37 |
| 3 |
Lake, Allyson C (12)
|
FGCSSC |
43.62 |
41.86 |
-1.76 |
| 4 |
Escobar, Genesis (11)
|
FGSOFL |
42.66 |
42.09 |
-0.57 |
| 5 |
Nanavati, Radhika K (11)
|
FGCSSC |
44.41 |
42.47 |
-1.94 |
| 6 |
Concho, Camila C (11)
|
FGTSA |
45.73 |
43.01 |
-2.72 |
| 7 |
Wood, Nikki S (11)
|
FGPOMP |
44.95 |
43.66 |
-1.29 |
| 8 |
Cupid, Chloe F (11)
|
FGPST |
44.20 |
43.79 |
-0.41 |
| 9 |
Sorokina, Daria (12)
|
FGHAT |
46.42 |
44.85 |
-1.57 |
| 10 |
Henry, Teryn (12)
|
FGTSA |
45.84 |
45.00 |
-0.84 |
| 11 |
Driscoll, Annelise N (12)
|
FGPCS |
|
45.38 |
- |
| 12 |
Navarro, Isadora G (12)
|
FGSOFL |
47.38 |
45.54 |
-1.84 |
| 13 |
Ford, Andie C (11)
|
FGSFTL |
45.89 |
45.65 |
-0.24 |
| 14 |
Yin, Darly (11)
|
FGSF |
53.70 |
46.98 |
-6.72 |
| 15 |
McComie-Waterman, An Alysse (11)
|
FGTSA |
51.05 |
47.30 |
-3.75 |
| 16 |
Shivakumar, Roshini (12)
|
FGSOFL |
46.72 |
47.88 |
1.16 |
| 17 |
Ortega, Shany C (11)
|
FGPST |
50.43 |
47.96 |
-2.47 |
| 18 |
Berding, Gabriela (11)
|
FGSFTL |
51.35 |
48.37 |
-2.98 |
| 19 |
Peterson, Andrea L (12)
|
FGCSSC |
50.44 |
49.60 |
-0.84 |
| 20 |
Marcano, Gabrielle E (11)
|
FGYSF |
|
49.70 |
- |
| 21 |
Thomas, Morgan A (11)
|
FGTSA |
59.46 |
50.08 |
-9.38 |
| 22 |
Porras, Sarah N (11)
|
FGAZFL |
52.42 |
51.21 |
-1.21 |
| 23 |
O'Mara, Caitlin K (11)
|
FGCSSC |
51.73 |
52.72 |
0.99 |
| 24 |
O'Connor, Claire C (11)
|
FGCSSC |
1:02.75 |
54.99 |
-7.76 |
| 25 |
Fohr, Kaitlyn E (11)
|
FGSF |
|
57.21 |
- |
|
Pozhydaieva, Polina (11)
|
FGSFTL |
|
NS |
- |
|
Lewis, Gel'lesa D (12)
|
FGSOFL |
42.24 |
NS |
- |
|
Ricardez, Maya G (11)
|
FGPOMP |
|
NS |
- |
|
Croasdale, Skylar K (12)
|
FGCSSC |
42.53 |
NS |
- |