| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Viera, Isabel (10)
|
FGMACM |
33.53 |
30.90 |
-2.63 |
| 2 |
Perez, Maria C (10)
|
FGMWA |
36.53 |
31.60 |
-4.93 |
| 3 |
Seoane, Carolina A (10)
|
FGMACM |
35.27 |
33.73 |
-1.54 |
| 4 |
Viera, Andrea (10)
|
FGMACM |
41.25 |
36.08 |
-5.17 |
| 5 |
Altamura, Benedetta R (10)
|
FGPST |
41.17 |
36.42 |
-4.75 |
| 6 |
Karachun, Daryna (10)
|
FGYSF |
34.57 |
36.95 |
2.38 |
| 7 |
Moreno, Antonia (9)
|
FGGRSC |
38.35 |
37.35 |
-1.00 |
| 8 |
Montenegro, Makayla E (9)
|
FGMACM |
36.58 |
37.64 |
1.06 |
| 9 |
Souza, Gabriela L (10)
|
FGGRSC |
46.43 |
40.81 |
-5.62 |
| 10 |
Almodovar, Lauren S (10)
|
FGGRSC |
47.02 |
41.85 |
-5.17 |
| 11 |
Ramos Miret, Carolina (9)
|
FGMACM |
|
43.74 |
- |
| 12 |
Suarez, Ava B (10)
|
FGYSF |
44.66 |
44.13 |
-0.53 |
| 13 |
Dabus, Rafaela A (9)
|
FGGRSC |
|
44.45 |
- |
| 14 |
Prigmore, Lauren (9)
|
FGMWA |
|
51.44 |
- |
| 15 |
Lenz Cesar, Maria Luiza B (9)
|
FGGRSC |
|
57.40 |
- |
|
Chacon, Kayla M (10)
|
FGSF |
|
DQ |
- |