| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Seoane, Carolina A (10)
|
FGMACM |
35.71 |
33.55 |
-2.16 |
| 2 |
Viera, Isabel (10)
|
FGMACM |
36.02 |
34.16 |
-1.86 |
| 3 |
Wolfe, Ava R (10)
|
FGMWA |
34.31 |
36.64 |
2.33 |
| 4 |
Karachun, Daryna (10)
|
FGYSF |
38.13 |
39.19 |
1.06 |
| 5 |
Viera, Andrea (10)
|
FGMACM |
42.24 |
40.60 |
-1.64 |
| 6 |
Rodriguez, Roxan (10)
|
FGMWA |
|
40.76 |
- |
| 7 |
Altamura, Benedetta R (10)
|
FGPST |
42.50 |
43.32 |
0.82 |
| 8 |
Roldan, Arianna * (9)
|
FGGRSC |
|
43.35 |
- |
| 9 |
Ramos Miret, Carolina (9)
|
FGMACM |
|
43.68 |
- |
| 10 |
Di Sarno, Naiyla F (9)
|
FGMWA |
51.93 |
43.74 |
-8.19 |
| 11 |
Almodovar, Lauren S (10)
|
FGGRSC |
47.38 |
44.53 |
-2.85 |
| 12 |
Suarez, Ava B (10)
|
FGYSF |
43.43 |
45.50 |
2.07 |
| 13 |
Dabus, Rafaela A (9)
|
FGGRSC |
50.18 |
46.29 |
-3.89 |
| 14 |
Prigmore, Lauren (9)
|
FGMWA |
52.48 |
50.34 |
-2.14 |
|
Lenz Cesar, Maria Luiza B (9)
|
FGGRSC |
53.30 |
DQ |
- |
|
Chaison, Molly A (10)
|
FGMWA |
43.83 |
NS |
- |