| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smyers, Molly E (16)
|
AMANSC |
2:28.88 |
2:28.50 |
-0.38 |
| 2 |
Thomas, Jewel A (15)
|
AMNHA |
2:24.06 |
2:33.61 |
9.55 |
| 3 |
Shaffer, Elizabeth L (14)
|
AMNHA |
2:28.77 |
2:33.68 |
4.91 |
| 4 |
Craig, Kendall A (16)
|
AMSVSC |
2:33.68 |
2:35.62 |
1.94 |
| 5 |
Kisow, Olivia P (16)
|
AMANSC |
2:31.43 |
2:36.45 |
5.02 |
| 6 |
Dietz, Ria E (15)
|
AMSVSC |
2:37.04 |
2:44.76 |
7.72 |
| 7 |
Tekelenburg, Emily (18)
|
AMSVSC |
2:57.24 |
2:49.49 |
-7.75 |
| 8 |
Dicken, Felicity G (14)
|
AMNHA |
3:20.01 |
2:50.74 |
-29.27 |
| 9 |
Bedell, Kristen L (16)
|
AMSVSC |
2:55.74 |
2:54.29 |
-1.45 |
| 10 |
Wilson, Alissa L (13)
|
AMSVSC |
3:10.05 |
2:57.01 |
-13.04 |
| 11 |
Sutterlin, Danielle M (15)
|
AMHD |
2:57.11 |
2:57.55 |
0.44 |
| 12 |
An, Emma K (13)
|
AMHD |
2:59.44 |
3:01.56 |
2.12 |
| 13 |
Dube, Madeline K (13)
|
AMNHA |
3:04.25 |
3:03.79 |
-0.46 |
| 14 |
Majors, Allison E (15)
|
AMRESY |
3:20.01 |
3:04.26 |
-15.75 |
| 15 |
Bedell, Lauren E (16)
|
AMSVSC |
2:55.74 |
3:05.07 |
9.33 |
| 16 |
Gifford, Adelie T (14)
|
AMSVSC |
3:06.18 |
3:11.32 |
5.14 |
| 17 |
Walko, Paige M (14)
|
AMSVSC |
3:23.47 |
3:32.49 |
9.02 |
|
Spiess, Emma E (13)
|
AMHD |
3:05.66 |
DQ |
- |