| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simonetti, Lucia M (10)
|
AMANSC |
44.12 |
43.38 |
-0.74 |
| 2 |
Masztak, Evey A (10)
|
AMANSC |
47.13 |
45.13 |
-2.00 |
| 3 |
Guan, Fiona (9)
|
AMANSC |
50.74 |
46.89 |
-3.85 |
| 4 |
Powell, Abigail C (10)
|
AMANSC |
52.83 |
49.03 |
-3.80 |
| 5 |
Dudkowski, Alina G (10)
|
AMPRA |
53.52 |
51.70 |
-1.82 |
| 6 |
Hollenbeck, Mackenzie E (9)
|
AMANSC |
54.18 |
52.89 |
-1.29 |
| 7 |
Emekter, Selin E (10)
|
AMANSC |
56.57 |
53.87 |
-2.70 |
| 8 |
Bhonsale, Siya (10)
|
AMANSC |
57.17 |
54.78 |
-2.39 |
| 9 |
Drozd, Emily M (9)
|
AMANSC |
58.89 |
56.89 |
-2.00 |
|
Bowman, Brooke A (7)
|
AMANSC |
1:11.21 |
DQ |
- |
|
Maravich, Avery R (7)
|
AMANSC |
1:02.87 |
DQ |
- |
|
Miller, Cora J (10)
|
AMANSC |
53.90 |
NS |
- |
|
Pomirleanu, Anamaria (10)
|
AMPRA |
47.35 |
NS |
- |