| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Katie (12)
|
MRYMID |
34.14 |
32.50 |
-1.64 |
| 2 |
Stidolph, Katherine E (11)
|
CTZEUS |
34.52 |
32.99 |
-1.53 |
| 3 |
Gilbertson, Rhianna S (11)
|
CTZEUS |
34.00 |
34.04 |
0.04 |
| 4 |
Levitt, Hannah R (11)
|
CTZEUS |
36.53 |
36.32 |
-0.21 |
| 5 |
Malionek, Zoe M (11)
|
MRCFB |
38.92 |
38.26 |
-0.66 |
| 6 |
Sharpe, Ashley B (11)
|
MRUN-K |
39.49 |
39.57 |
0.08 |
| 7 |
Richards, Aryel N (11)
|
CTZEUS |
|
40.22 |
- |
| 8 |
DePasquale, Jenna K (11)
|
CTZEUS |
40.45 |
42.38 |
1.93 |
| 9 |
Fedor, Maggie I (11)
|
CTZEUS |
44.69 |
43.65 |
-1.04 |
| 10 |
Reed, Nia N (12)
|
MRKBM |
46.24 |
45.58 |
-0.66 |
| 11 |
Pepper-Jones, BB (11)
|
MRCFB |
|
46.37 |
- |
| 12 |
Ramos, Samantha (12)
|
MRKBM |
46.25 |
47.20 |
0.95 |
| 13 |
Vargas, Jadyn S (11)
|
MRKBM |
51.74 |
48.24 |
-3.50 |
| 14 |
Radick, Susannah (11)
|
MRCFB |
54.68 |
48.81 |
-5.87 |
|
Popielarz, Victoria A (12)
|
CTZEUS |
38.20 |
DQ |
- |
|
Loor- Steinberg, Thalia (12)
|
NJMALT |
47.54 |
NS |
- |
|
Moy, Xena E (12)
|
CTZEUS |
|
NS |
- |