| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ray, Nidhi (8)
|
MREAST |
46.24 |
45.11 |
-1.13 |
| 2 |
Aikman, Saskia M (8)
|
MRYMID |
|
47.44 |
- |
| 3 |
Amen, Bianca J (8)
|
MRYFD |
|
48.84 |
- |
| 4 |
Xiao, Lucia (8)
|
MRYFD |
|
50.24 |
- |
| 5 |
Moore, Cora M (8)
|
MRSMC |
46.99 |
50.32 |
3.33 |
| 6 |
Martinez-Jimenez, Alba (8)
|
MRYMID |
56.67 |
50.80 |
-5.87 |
| 7 |
Carvente, Ashley (8)
|
CTZEUS |
55.44 |
51.03 |
-4.41 |
| 8 |
Rodriguez, Melanie (8)
|
MRYMID |
51.30 |
52.05 |
0.75 |
| 9 |
Burriss, Leah S (8)
|
MREAST |
51.90 |
52.31 |
0.41 |
| 10 |
Losic, Ana (8)
|
MRYMID |
|
52.43 |
- |
| 11 |
Fusco, Charlotte G (8)
|
MRGAEL |
1:02.33 |
52.90 |
-9.43 |
| 12 |
Tello, Samantha (8)
|
MRSPAR |
57.57 |
53.30 |
-4.27 |
| 13 |
Hyatt, Caitlyn M (8)
|
MRYMID |
|
53.74 |
- |
| 14 |
Levy, Sophia (8)
|
MRYMID |
|
54.27 |
- |
| 15 |
McKibben, Tyler l (8)
|
MRYFD |
56.31 |
55.22 |
-1.09 |
| 16 |
Kim-Yalter, Sophie R (8)
|
MRYFD |
|
55.23 |
- |
| 17 |
Pastore, Caroline G (8)
|
MRSPAR |
56.29 |
55.56 |
-0.73 |
| 18 |
Ceccarini, Isabella G (8)
|
MRYFD |
54.21 |
56.82 |
2.61 |
| 19 |
Vanover, Danielle R (8)
|
MREAST |
52.70 |
56.89 |
4.19 |
| 20 |
Ross, Leila (8)
|
MRYFD |
|
56.96 |
- |
| 21 |
Henriquez, Zayra (8)
|
CTZEUS |
55.86 |
57.77 |
1.91 |
| 22 |
Simon, Charlotte B (8)
|
MRYFD |
|
58.08 |
- |
| 23 |
Fung, Maya M (8)
|
MRYFD |
|
59.83 |
- |
| 24 |
Lussie, Alexandra m (8)
|
MRYFD |
|
1:00.61 |
- |
| 25 |
Pavlonis, Savanna (8)
|
MRCFB |
50.32 |
1:01.06 |
10.74 |
| 26 |
Kopel, Romi D (8)
|
MRYFD |
59.60 |
1:01.23 |
1.63 |
| 27 |
Lemin, Juliana A (8)
|
MREAST |
1:10.33 |
1:01.30 |
-9.03 |
| 28 |
Enright, Delaney (8)
|
MRYFD |
|
1:01.69 |
- |
| 29 |
Freedman, Julia S (8)
|
MRYFD |
1:07.83 |
1:02.65 |
-5.18 |
| 30 |
Sorkin, Olivia J (8)
|
MRYMID |
|
1:02.79 |
- |
| 31 |
Hubell, Kate L (8)
|
MRYMID |
|
1:05.26 |
- |
| 32 |
Stauffer, Clara J (8)
|
MRYFD |
|
1:18.47 |
- |
|
Sorkin, Sophia R (8)
|
MRYMID |
|
DQ |
- |
|
Cunniffe, Isla G (8)
|
CTZEUS |
54.92 |
DQ |
- |