| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Settlemyre, Jeffrey P (18)
|
NCWOW |
50.97 |
53.06 |
2.09 |
| 2 |
Winchester, Austin O (17)
|
NCWOW |
51.05 |
53.24 |
2.19 |
| 3 |
Furbay, Patrick D (16)
|
NCWOW |
53.38 |
55.36 |
1.98 |
| 4 |
Sterrett, William J (15)
|
NCWOW |
53.00 |
57.19 |
4.19 |
| 5 |
Butner, Mark L (16)
|
NCWOW |
56.53 |
59.50 |
2.97 |
| 6 |
Luo, Matthew Z (16)
|
NCWOW |
58.97 |
1:00.63 |
1.66 |
| 7 |
Langtry, Ryan C (14)
|
NCWOW |
1:01.04 |
1:00.64 |
-0.40 |
| 8 |
Davis, Matthew C (16)
|
NCWOW |
1:00.01 |
1:00.65 |
0.64 |
| 9 |
Parker, William H (16)
|
UNAT |
55.06 |
1:01.55 |
6.49 |
| 10 |
Post, Cian D (13)
|
NCWOW |
1:06.74 |
1:02.75 |
-3.99 |
| 11 |
Setzer, Sean T (14)
|
NCWOW |
1:01.74 |
1:02.80 |
1.06 |
| 12 |
Stuck, Evan J (14)
|
NCWOW |
1:03.09 |
1:02.95 |
-0.14 |
| 13 |
Lyons, Frank E (16)
|
NCWOW |
1:01.88 |
1:03.39 |
1.51 |
| 14 |
Hyldahl, Cooper C (16)
|
NCWOW |
1:07.98 |
1:06.63 |
-1.35 |
| 15 |
Hughes, Jackson C (13)
|
NCWOW |
1:02.88 |
1:06.68 |
3.80 |
| 16 |
Caravaglio, Matthew B (14)
|
NCWOW |
1:15.87 |
1:09.23 |
-6.64 |
| 17 |
Eberhardt, Nathan L (14)
|
NCWOW |
1:11.94 |
1:11.30 |
-0.64 |
| 18 |
Ive, Jethro T (13)
|
NCWOW |
|
1:22.54 |
- |
| 19 |
Mejouev, Maxim (13)
|
NCWOW |
1:24.19 |
1:24.85 |
0.66 |