| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, Sophia W (8)
|
NEATST |
47.45 |
47.02 |
-0.43 |
| 2 |
Shea, Brynn L (8)
|
NEHAY |
54.87 |
47.62 |
-7.25 |
| 3 |
Cowan, Catherine L (8)
|
NEUSC |
52.00 |
48.51 |
-3.49 |
| 4 |
Garvey, Grace B (8)
|
NEHAY |
|
50.40 |
- |
| 5 |
Zhou, Philina (8)
|
NEUNUS |
1:35.85 |
50.73 |
-45.12 |
| 6 |
McGrath, Grace H (8)
|
NECRIM |
|
51.42 |
- |
| 7 |
Levari, Tamar T (8)
|
NECRIM |
55.68 |
56.44 |
0.76 |
| 8 |
Liu, Selena W (6)
|
NEATST |
|
1:04.88 |
- |
|
Bissonnette, Haley E (7)
|
NECRIM |
|
DQ |
- |
|
Mailloux, Gabrielle E (8)
|
NECRIM |
|
DQ |
- |
|
Field, Sophie R (8)
|
NEHAY |
|
DQ |
- |
|
Kang, Chloe H (7)
|
NEATST |
|
NS |
- |
|
Mack, Calais S (8)
|
NECRIM |
50.15 |
NS |
- |