| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jiang, Lily (10)
|
MRCENT |
51.31 |
45.43 |
-5.88 |
| 1 |
Gray, Naevia (10)
|
MRHES |
52.21 |
49.41 |
-2.80 |
| 2 |
Fried, Nica (10)
|
MRLIFE |
54.66 |
49.56 |
-5.10 |
| 3 |
Block, Olette Z (9)
|
MRUN |
54.06 |
50.22 |
-3.84 |
| 4 |
Walker, Lincoln M (10)
|
MRFREE |
50.30 |
50.23 |
-0.07 |
| 5 |
Herrera-Morales, Khiara C (10)
|
MRAPEX |
51.29 |
50.32 |
-0.97 |
| 6 |
Moshette, Savannah (10)
|
MRNFS |
56.03 |
51.13 |
-4.90 |
| 7 |
Healy, Sophie (9)
|
MRLIFE |
51.97 |
51.52 |
-0.45 |
| 8 |
Townley, Mira (10)
|
MRMAKO |
54.49 |
52.50 |
-1.99 |
| 9 |
Barbier, Leah (10)
|
MRAPEX |
54.83 |
53.59 |
-1.24 |
| 10 |
Hewitt, Melody (10)
|
MRHES |
57.50 |
53.76 |
-3.74 |
| 11 |
Daniel, Chloe (9)
|
MRHES |
57.60 |
54.04 |
-3.56 |
| 12 |
Diamond, Phoebe (10)
|
MRAPEX |
1:00.99 |
54.36 |
-6.63 |
| 13 |
Yuan, Penelope (9)
|
MRCENT |
59.41 |
54.42 |
-4.99 |
| 14 |
Slomko, Hannah S (10)
|
MRKBM |
50.93 |
55.24 |
4.31 |
| 15 |
Tsao-Chen, Sylvia (9)
|
MRLIFE |
58.17 |
55.46 |
-2.71 |
| 16 |
Oyola, Emmie G (10)
|
MRKBM |
51.41 |
56.31 |
4.90 |
| 17 |
Deng, Morgan Z (10)
|
MRHES |
58.06 |
56.32 |
-1.74 |
| 18 |
Padilla, Khloe (10)
|
MRKBM |
1:00.63 |
56.65 |
-3.98 |
| 19 |
Whelan, Nora M (9)
|
MRBKR |
59.38 |
58.61 |
-0.77 |
| 20 |
Delinois, Ruby (9)
|
MRRED |
1:06.02 |
1:01.05 |
-4.97 |
| 21 |
Verdezoto Y., Sammy J (10)
|
MRRED |
1:12.12 |
1:12.74 |
0.62 |
|
DeVonish, Tori R (9)
|
MRKBM |
1:07.99 |
DQ |
- |
|
Felipe, Layla M (10)
|
MRKBM |
1:03.54 |
DQ |
- |
|
Hinds, Ella (10)
|
MRKBM |
1:03.92 |
DQ |
- |
|
Davidson, Ella S (9)
|
MRMAKO |
1:02.53 |
DQ |
- |
|
Verbon Font, Julia V (10)
|
MRCENT |
58.04 |
DQ |
- |
|
Sarajlic, Lena A (9)
|
MRCENT |
56.99 |
DQ |
- |