| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
James, Grace A (10)
|
MAUN-0 |
34.86 |
35.94 |
1.08 |
| 2 |
Kissling, Abigail M (10)
|
MAGMAC |
|
38.04 |
- |
| 3 |
Laure, Kendall A (10)
|
MACYA |
38.45 |
38.55 |
0.10 |
| 4 |
Foley, Lily E (10)
|
MAHAC |
41.89 |
42.29 |
0.40 |
| 5 |
Virabyan, Angelika J (10)
|
MAHAC |
48.04 |
44.49 |
-3.55 |
| 6 |
Reeves, Leah M (10)
|
MAKA |
47.57 |
46.95 |
-0.62 |
| 7 |
Steinkirchner, Kaitlin P (10)
|
MACYA |
49.69 |
48.81 |
-0.88 |
| 8 |
Zeigler, Isabella J (10)
|
MACYA |
47.90 |
49.12 |
1.22 |
| 9 |
Brunner, Emma J (10)
|
MACYA |
51.15 |
51.87 |
0.72 |
| 10 |
Bistline, Rebecca R (10)
|
MASHIP |
|
51.88 |
- |
| 11 |
Stefanelli, Isabella L (10)
|
MAUN-0 |
56.53 |
55.70 |
-0.83 |
| 12 |
Bhonde, Jheel P (10)
|
MACYA |
1:03.05 |
1:01.11 |
-1.94 |
|
Fiore, Mariella S (10)
|
MAMUHL |
34.20 |
DQ |
- |
|
Novak, Sascha L (10)
|
MAUN-0 |
|
NS |
- |