| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pesek, Alice (9)
|
LECEA |
50.84 |
48.02 |
-2.82 |
| 2 |
Rosenbaum, Katherine A (8)
|
LEGO |
48.06 |
48.64 |
0.58 |
| 3 |
Shantery, Brooke L (9)
|
LEGO |
50.99 |
49.82 |
-1.17 |
| 4 |
Potts, Sophia E (9)
|
LEPS |
51.99 |
50.15 |
-1.84 |
| 5 |
Henning, Hayley M (9)
|
LEVSC |
48.11 |
52.71 |
4.60 |
| 6 |
Birmingham, Leighton E (9)
|
LEPA |
56.81 |
55.00 |
-1.81 |
| 7 |
Mann, Skylar A (9)
|
LEGO |
54.65 |
57.52 |
2.87 |
| 8 |
Fritz, Alexa J (9)
|
LEPS |
59.78 |
57.54 |
-2.24 |
| 9 |
Nappo, Tessa G (8)
|
LEPA |
58.84 |
1:02.29 |
3.45 |
| 10 |
Waldman, Payton R (8)
|
LEPAC |
|
1:14.02 |
- |
| 11 |
Roberts, Emma R (7)
|
LEPA |
|
1:17.26 |
- |
|
Persinger, Madeleine D (9)
|
LEPWRS |
|
DQ |
- |