| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fields, Michael C (17)
|
NTTRAC |
49.18 |
48.01 |
-1.17 |
| 2 |
Sims, Kaden A (17)
|
NTMTRO |
52.39 |
49.58 |
-2.81 |
| 3 |
Eberhard, Mark W (17)
|
NTMTRO |
51.95 |
49.69 |
-2.26 |
| 4 |
Sharp, Ryan A (18)
|
NTMTRO |
49.25 |
50.42 |
1.17 |
| 5 |
Oster, Davis A (17)
|
NTTRAC |
51.08 |
50.66 |
-0.42 |
| 6 |
Green, Michael R (16)
|
NTMTRO |
53.43 |
51.00 |
-2.43 |
| 7 |
Baker, Griffin B (15)
|
NTTRAC |
51.42 |
51.21 |
-0.21 |
| 8 |
McCullough, Hayden C (16)
|
NTTRAC |
52.22 |
52.09 |
-0.13 |
| 9 |
Beard, Macen M (15)
|
NTMTRO |
|
53.15 |
- |
| 10 |
Cleveland, Ryan L (15)
|
NTTRAC |
55.47 |
53.63 |
-1.84 |
| 11 |
Kozsuch, Kaden L (16)
|
NTMTRO |
54.60 |
54.48 |
-0.12 |
| 12 |
Tanksley, William R (15)
|
NTTRAC |
|
54.69 |
- |
| 13 |
Petty, Brady S (17)
|
NTTRAC |
52.25 |
54.88 |
2.63 |
| 14 |
Rose, Titus H (16)
|
NTMTRO |
57.66 |
57.90 |
0.24 |
| 15 |
Scott, Matthew D (16)
|
NTMTRO |
57.32 |
58.93 |
1.61 |
| 16 |
Richbourg, Bradley W (16)
|
NTMTRO |
|
59.80 |
- |
| 17 |
Watters, Micah J (16)
|
NTTRAC |
|
1:01.55 |
- |
| 18 |
Foster, Gabriel L (15)
|
NTMTRO |
|
1:05.91 |
- |