| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Witte, Dana C (14)
|
NIWEBS |
59.60 |
1:00.20 |
0.60 |
| 2 |
Sokolowski, Suzanne N (14)
|
NIWEBS |
1:01.04 |
1:00.89 |
-0.15 |
| 3 |
Salmon, Paige R (14)
|
NITWST |
1:00.99 |
1:02.63 |
1.64 |
| 4 |
Jones, Katherine E (14)
|
NITWST |
1:05.13 |
1:02.92 |
-2.21 |
| 5 |
Sylvestri, Sofia I (14)
|
NIPACK |
1:01.97 |
1:05.27 |
3.30 |
| 6 |
Worrall, Alexis R (14)
|
NIFAST |
1:08.07 |
1:06.78 |
-1.29 |
| 7 |
Hahn, Elizabeth P (14)
|
NIWEBS |
1:06.93 |
1:07.01 |
0.08 |
| 8 |
Upcraft, Maya E (14)
|
NIMETS |
1:10.74 |
1:08.15 |
-2.59 |
| 9 |
Wiegand, Bailey M (14)
|
NITTSC |
1:07.95 |
1:10.43 |
2.48 |
| 10 |
Flash, Maya I (14)
|
NITTSC |
1:04.91 |
1:10.76 |
5.85 |
| 11 |
Cavanaugh, Erin M (14)
|
NITTSC |
1:11.46 |
1:10.90 |
-0.56 |
| 12 |
Musterait, Madeline A (14)
|
NITWST |
1:13.90 |
1:15.64 |
1.74 |