| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zani, Samuel A (15)
|
NIIRON |
24.83 |
24.20 |
-0.63 |
| 2 |
Kibler, Clayton M (15)
|
NITWST |
25.36 |
24.59 |
-0.77 |
| 3 |
Sapio, Luke T (15)
|
NITWST |
25.20 |
24.80 |
-0.40 |
| 4 |
Markham, Rowan M (15)
|
NIFAST |
25.34 |
24.94 |
-0.40 |
| 5 |
Kibler, Seth A (15)
|
NITWST |
25.14 |
25.16 |
0.02 |
| 6 |
Palacio, Gabriel D (15)
|
NIHAWK |
26.71 |
25.68 |
-1.03 |
| 6 |
O'Shea, Connor J (15)
|
NIFAST |
25.81 |
25.68 |
-0.13 |
| 8 |
Hand, Gavin J (15)
|
NIGVST |
25.66 |
25.80 |
0.14 |
| 9 |
Christopher, Nicholas W (15)
|
NIHAMB |
26.36 |
26.41 |
0.05 |
| 10 |
Mueller, Christopher J (15)
|
NISDSC |
27.77 |
28.19 |
0.42 |
|
Ocker, Joe M (15)
|
NIMETS |
27.06 |
NS |
- |