| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
von Holtz, Alexa L (12)
|
NIMETS |
25.83 |
26.79 |
0.96 |
| 2 |
Nguyen, Vivian A (12)
|
NIWEBS |
27.93 |
28.21 |
0.28 |
| 3 |
Sauer, Emma L (12)
|
NIHAMB |
29.16 |
28.88 |
-0.28 |
| 4 |
Bedford, Ainsley N (12)
|
NIWEBS |
30.20 |
29.42 |
-0.78 |
| 5 |
Griffin, Haley A (12)
|
NITTSC |
29.44 |
29.77 |
0.33 |
| 6 |
Kassirer, Natalie G (12)
|
NITWST |
30.77 |
29.90 |
-0.87 |
| 7 |
Martin, Lauren G (12)
|
NISDSC |
30.94 |
31.60 |
0.66 |
| 8 |
Oneil, Isabel D (12)
|
NIWEBS |
32.71 |
32.44 |
-0.27 |
| 9 |
Brandes, Tiernee S (12)
|
NIGVST |
31.15 |
33.15 |
2.00 |
| 10 |
Carey, Madison H (12)
|
NIIRON |
33.17 |
33.27 |
0.10 |
| 11 |
Johnson, Ila A (12)
|
NIIRON |
36.05 |
34.89 |
-1.16 |
| 12 |
Mancuso, Gabriella J (12)
|
NIHAMB |
35.48 |
35.24 |
-0.24 |
| 13 |
Minnigh, Christina N (12)
|
NIPACK |
36.05 |
36.99 |
0.94 |
| 14 |
Minnigh, Ella N (12)
|
NIPACK |
34.61 |
37.19 |
2.58 |
| 15 |
Campbell, Brooklyn M (12)
|
NIVICT |
37.70 |
38.67 |
0.97 |
|
Goodridge, Jenna L (12)
|
NIWEBS |
29.26 |
NS |
- |