| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Stella V (10)
|
NTNTN |
31.88 |
32.75 |
0.87 |
| 2 |
Rockafellow, Charlie E (9)
|
NTNTN |
43.26 |
38.70 |
-4.56 |
| 3 |
Smetana, Shelby T (8)
|
NTNTN |
43.43 |
43.08 |
-0.35 |
| 4 |
Dukes, Kameron C (10)
|
NTNTN |
44.70 |
43.36 |
-1.34 |
| 5 |
Palmer, Elizabeth J (8)
|
NTNTN |
47.09 |
43.59 |
-3.50 |
| 6 |
Walser, Georgia H (10)
|
NTNTN |
49.64 |
43.62 |
-6.02 |
| 7 |
Franco, Aliana M (8)
|
NTNTN |
47.10 |
44.26 |
-2.84 |
| 8 |
Weilert, Chandler R (9)
|
NTNTN |
49.16 |
47.02 |
-2.14 |
| 9 |
Frost, Ava M (9)
|
NTNTN |
|
49.72 |
- |
| 10 |
Nguyen, Emma H (9)
|
NTNTN |
50.75 |
49.75 |
-1.00 |
| 11 |
Kizer, Claire R (8)
|
NTNTN |
|
49.86 |
- |
| 12 |
Bartz, Annika M (8)
|
NTNTN |
|
51.58 |
- |
|
Klopfenstein, Katelyn J (8)
|
NTNTN |
|
DQ |
- |
|
McNary, Kameron K (10)
|
NTNTN |
|
DQ |
- |