| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crye, Joleigh C (12)
|
NIFAST |
26.89 |
26.52 |
-0.37 |
| 2 |
Pignatelli, Maria L (12)
|
NIUNAC |
28.42 |
27.43 |
-0.99 |
| 3 |
Figueroa, Marissa J (11)
|
NICAGO |
29.75 |
29.37 |
-0.38 |
| 4 |
Williams, Taegan S (12)
|
NIWAAC |
28.61 |
29.42 |
0.81 |
| 5 |
Iosub, Lorena M (12)
|
NIUNAC |
29.72 |
29.79 |
0.07 |
| 6 |
Gill-Martiny, Lily M (11)
|
NIUNAC |
30.54 |
29.93 |
-0.61 |
| 7 |
Bush, Sarah (12)
|
NICAGO |
30.41 |
31.15 |
0.74 |
| 8 |
Bucci, Mikayla (12)
|
NICVSC |
31.13 |
31.19 |
0.06 |
| 9 |
Olsian, Ava C (11)
|
NIUNAC |
32.25 |
31.75 |
-0.50 |
| 10 |
Vascoe, Delaney M (12)
|
NIWAAC |
31.34 |
32.58 |
1.24 |
| 11 |
Barnes, Marannda J (12)
|
ADSIDN |
32.71 |
33.20 |
0.49 |
| 12 |
Gardner, Raven L (12)
|
NIWAAC |
31.34 |
33.36 |
2.02 |
| 13 |
Sharpsteen, Willow (12)
|
NIWAAC |
33.60 |
33.38 |
-0.22 |
| 14 |
Hope, Kate E (11)
|
NICVSC |
35.40 |
34.70 |
-0.70 |
| 15 |
Marshall, Emily V (11)
|
NIWAAC |
35.95 |
34.77 |
-1.18 |
| 16 |
Brady, Kelsey R (11)
|
NIUNAC |
35.47 |
35.85 |
0.38 |
| 17 |
Nickerson, Sarah (11)
|
NICVSC |
39.17 |
36.90 |
-2.27 |
| 18 |
Reese, Reagan a (11)
|
NIGGST |
47.03 |
36.97 |
-10.06 |
| 19 |
Austin, Victoria A (11)
|
NICAGO |
45.11 |
37.68 |
-7.43 |
| 20 |
Rule, Lilyan M (12)
|
NIUNAC |
37.65 |
38.36 |
0.71 |
| 21 |
Kneer, Lauren A (11)
|
NIUNAC |
39.78 |
38.45 |
-1.33 |
| 22 |
Richeda, Olivia J (11)
|
NIUNAC |
40.87 |
39.26 |
-1.61 |
| 23 |
Neubert, Elaina C (11)
|
ADSIDN |
46.27 |
39.50 |
-6.77 |
| 24 |
Bunke, Nicole P (12)
|
NIWAAC |
38.69 |
40.87 |
2.18 |
| 25 |
Santa Croce, Olivia L (11)
|
NICVSC |
36.84 |
42.85 |
6.01 |
| 26 |
Ottoson, Midian R (11)
|
ADSIDN |
|
45.56 |
- |
| 27 |
Cantrell, Logan E (11)
|
NIWAAC |
40.67 |
45.71 |
5.04 |
|
Scales, Courtney R (12)
|
NICAGO |
34.34 |
NS |
- |