| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ohtani, Mizuki (9)
|
MDCAA |
3:20.06 |
3:11.07 |
-8.99 |
| 2 |
Cason, Ameenah B (10)
|
MDBCSC |
3:20.22 |
3:18.51 |
-1.71 |
| 3 |
Folcomer, Tiffany P (10)
|
MAYY |
3:16.01 |
3:18.59 |
2.58 |
| 4 |
Moore, Chloe O (10)
|
MAYY |
3:34.88 |
3:28.32 |
-6.56 |
| 5 |
Trang, Kimberley T (9)
|
PVRMSC |
3:40.61 |
3:29.09 |
-11.52 |
| 6 |
Miller, Brianna E (9)
|
MAYY |
3:43.31 |
3:39.79 |
-3.52 |
| 7 |
Cahill, Kimberly R (10)
|
MDYRAC |
4:26.06 |
3:40.25 |
-45.81 |
| 8 |
Sumpter, Emerson (9)
|
MDYRAC |
|
3:43.96 |
- |
| 9 |
St. Pierre, Deanna R (10)
|
MDJCC |
3:38.71 |
3:44.13 |
5.42 |
| 10 |
Snyder, Rebecca M (10)
|
MAYY |
3:41.48 |
3:44.32 |
2.84 |
| 11 |
Pingaro, Regen O (9)
|
MDCAA |
|
3:45.76 |
- |
| 12 |
Gilhooly, Jenna E (10)
|
MDMAC |
|
4:01.75 |
- |
| 13 |
Johns, Aryon U (9)
|
MDBCSC |
5:05.55 |
4:07.90 |
-57.65 |
| 14 |
Tovar, Eloisa (8)
|
MASAKA |
|
4:14.50 |
- |
| 15 |
Drinan, Danielle (8)
|
MDBAAC |
3:59.55 |
4:14.78 |
15.23 |
| 16 |
Akhtarkhavari, Arshin (9)
|
MDCAA |
|
4:25.19 |
- |
| 17 |
Parton, Olivia L (8)
|
MDRAC |
|
4:34.05 |
- |
| 18 |
Ellenbecker, Vivienne N (9)
|
MDBAAC |
5:11.54 |
4:34.73 |
-36.81 |
| 19 |
Waldt, Sara G (8)
|
MDBAAC |
4:36.70 |
4:40.67 |
3.97 |
| 20 |
Smith, Kennedy G (10)
|
MDBCSC |
|
4:47.37 |
- |
|
Krownapple, Josephine M (9)
|
MDCAA |
|
NS |
- |
|
Nishnianidze, Anna (9)
|
PVRMSC |
3:29.97 |
NS |
- |