| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wise, Isabella H (17)
|
MAYY |
|
39.45 |
- |
| 2 |
Marston, Araby S (15)
|
MAYY |
|
39.80 |
- |
| 3 |
Wickham, Amelia C (17)
|
MDBAAC |
40.68 |
40.67 |
-0.01 |
| 4 |
Hunt, Katelyn E (16)
|
MAYY |
41.68 |
40.69 |
-0.99 |
| 5 |
Williams, Arianna S (15)
|
NEBGNE |
39.54 |
41.91 |
2.37 |
| 6 |
Solomich, Anna J (15)
|
PVRMSC |
41.91 |
42.72 |
0.81 |
| 7 |
McCleary, Taylor D (17)
|
MDJCC |
43.90 |
43.10 |
-0.80 |
| 8 |
Kosiorowski, Lauren N (15)
|
PVRMSC |
|
44.93 |
- |
| 9 |
Dobrzykowski, Natalie E (16)
|
PVRMSC |
|
45.05 |
- |
| 9 |
Entner, Rebecca F (17)
|
MDBAAC |
43.96 |
45.05 |
1.09 |
| 11 |
Todd, Sabrine M (18)
|
MDJCC |
45.70 |
46.43 |
0.73 |
| 12 |
Wallace, Jordan P (16)
|
PVRMSC |
40.61 |
46.56 |
5.95 |
| 13 |
Davies, Emily M (15)
|
MDHSST |
48.03 |
47.09 |
-0.94 |
| 14 |
Pflieger, Zoe E (17)
|
MDBCSC |
48.85 |
50.96 |
2.11 |
| 15 |
Muhammad, Ndera S (15)
|
MDBCSC |
|
1:13.42 |
- |
|
Friedman, Jensen L (16)
|
MDEST |
40.22 |
NS |
- |
|
Krownapple, Josephine M (9)
|
MDCAA |
1:01.96 |
NS |
- |
|
Devine, Molly K (16)
|
MAYY |
|
NS |
- |
|
Grant, Jewel C (15)
|
MDBCSC |
|
NS |
- |
|
Peltzer, Sophie E (15)
|
MAYY |
|
NS |
- |
|
Walsh, Keely M (15)
|
MAYY |
|
NS |
- |