| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bischof, Jaina L (10)
|
ADCLRK |
39.61 |
36.23 |
-3.38 |
| 2 |
Meka, Olivia G (9)
|
ADCANA |
37.59 |
39.71 |
2.12 |
| 3 |
Fuller, Grace V (10)
|
ADCLRK |
44.60 |
40.36 |
-4.24 |
| 4 |
Kapusniak, Claire G (10)
|
ADCLRK |
48.59 |
43.06 |
-5.53 |
| 5 |
Kaltenbach, Mia C (10)
|
ADCLRK |
|
44.17 |
- |
| 6 |
Raymond, Gabriella M (10)
|
ADCLRK |
1:33.35 |
44.63 |
-48.72 |
| 7 |
Adam, Bianca J (9)
|
ADCLRK |
|
45.93 |
- |
| 8 |
Phillips, Tara M (10)
|
ADCLRK |
49.93 |
46.38 |
-3.55 |
| 9 |
Crewell, Brynn R (10)
|
ADSVP |
57.00 |
47.00 |
-10.00 |
| 10 |
Litzner, Charlotte L (10)
|
ADSVP |
47.00 |
47.58 |
0.58 |
| 11 |
Frew, Emery P (9)
|
ADCANA |
51.86 |
51.77 |
-0.09 |
| 12 |
Patel, Arya S (9)
|
ADCLRK |
58.85 |
54.32 |
-4.53 |
| 13 |
Rios, Kylee-Lynn K (10)
|
ADAMST |
|
1:19.20 |
- |
|
Vroman, Ava G (9)
|
ADCANA |
|
NS |
- |
|
Scott, Isabella R (10)
|
ADSVP |
1:05.91 |
NS |
- |