| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Matthias, Jade A (10)
|
MAUN |
1:04.62 |
1:02.72 |
-1.90 |
| 2 |
Waslyn, Rylee E (10)
|
MAWAC |
1:04.81 |
1:03.01 |
-1.80 |
| 3 |
Marlin, Krista N (10)
|
MAGRA |
1:06.43 |
1:04.07 |
-2.36 |
| 4 |
Helms, Katherine P (10)
|
PVMAKO |
1:06.23 |
1:04.28 |
-1.95 |
| 5 |
Cover, Olivia N (10)
|
MAFSSC |
1:06.83 |
1:04.34 |
-2.49 |
| 6 |
Howley, Tess O (9)
|
MRLBA |
1:05.63 |
1:04.70 |
-0.93 |
| 7 |
Burton, Apolina M (10)
|
MAEPAC |
1:07.43 |
1:04.91 |
-2.52 |
| 8 |
Stramara, Gabrielle R (10)
|
MAFSSC |
1:06.48 |
1:05.31 |
-1.17 |
| 9 |
Cassidy, Megan E (10)
|
MAGRA |
1:07.68 |
1:05.32 |
-2.36 |
| 10 |
Foltz, Caroline A (10)
|
MADSC |
1:05.30 |
1:06.59 |
1.29 |
| 11 |
Turco, Lily M (10)
|
MAGRA |
1:07.97 |
1:07.31 |
-0.66 |
| 12 |
Simpson, Katherine A (10)
|
MALCY |
1:06.80 |
1:07.71 |
0.91 |
| 13 |
Agne, Kira W (9)
|
MAWAC |
1:07.59 |
1:07.82 |
0.23 |
| 14 |
Shui, Lilly Y (10)
|
MAPAY |
1:15.92 |
1:07.99 |
-7.93 |
| 15 |
Brandt, Mackenzie A (10)
|
MAWAC |
1:07.36 |
1:08.08 |
0.72 |
|
Mehta, Simran I (10)
|
MANPAC |
1:06.19 |
NS |
- |