| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mack, Katelyn G (12)
|
PVPV |
52.75 |
53.64 |
0.89 |
| 2 |
Thamm, Claudia M (12)
|
MAMA |
53.13 |
54.31 |
1.18 |
| 3 |
Standfield, Gabrielle R (12)
|
PVPV |
54.79 |
54.97 |
0.18 |
| 4 |
Gravereaux, Chelsea H (12)
|
MAMA |
54.96 |
55.11 |
0.15 |
| 5 |
Regenauer, Christiana M (12)
|
MRMETR |
53.74 |
55.37 |
1.63 |
| 6 |
Kopac, Allison N (12)
|
PVPV |
55.58 |
55.81 |
0.23 |
| 7 |
Competello, Madeline G (12)
|
MRMETR |
54.78 |
55.82 |
1.04 |
| 8 |
Thrall, Abigail (12)
|
CTCT |
56.05 |
55.84 |
-0.21 |
| 9 |
Saliba, Victoria A (12)
|
NJNJS |
55.63 |
55.90 |
0.27 |
| 10 |
Goettler, Laura B (12)
|
AMAM |
56.36 |
55.95 |
-0.41 |
| 11 |
Tuttle, Mackenzie M (12)
|
MRMETR |
54.46 |
55.98 |
1.52 |
| 12 |
Pierce, Summer M (12)
|
NINI |
55.31 |
56.08 |
0.77 |
| 13 |
Ramey, Abigail C (12)
|
AMAM |
56.45 |
56.13 |
-0.32 |
| 14 |
Famous, Caroline A (12)
|
MAMA |
54.94 |
56.30 |
1.36 |
| 15 |
Brinker, Mary E (12)
|
NINI |
56.36 |
56.64 |
0.28 |
| 16 |
Scott, Samantha (12)
|
CTCT |
55.62 |
56.73 |
1.11 |
| 17 |
Comaniciu, Alexandra (12)
|
NJNJS |
56.10 |
57.12 |
1.02 |
| 18 |
Chiu, Stephanie (12)
|
NJNJS |
56.55 |
57.22 |
0.67 |
| 19 |
Halsdorfer, Abby K (12)
|
NINI |
57.04 |
57.38 |
0.34 |
| 20 |
Ross, Leia (12)
|
AMAM |
56.46 |
57.45 |
0.99 |
| 21 |
Lindsey, Shannon (12)
|
CTCT |
56.65 |
58.09 |
1.44 |
| 22 |
Hartwich, Alexandra G (12)
|
ADAD |
58.45 |
59.79 |
1.34 |
| 23 |
Ecker, Emily D (11)
|
MEMAIN |
58.82 |
1:00.22 |
1.40 |
| 24 |
Digiovanni, Amaya M (11)
|
ADAD |
1:00.91 |
1:00.50 |
-0.41 |
| 25 |
Mahoney, Caroline E (12)
|
MEMAIN |
1:00.29 |
1:00.76 |
0.47 |
| 26 |
Conway, Mary K (12)
|
ADAD |
59.15 |
1:01.79 |
2.64 |
| 27 |
Park, Jaehee (11)
|
MEMAIN |
1:00.45 |
1:02.35 |
1.90 |
| 28 |
Doughty, Emma J (12)
|
NINI |
1:03.61 |
1:02.37 |
-1.24 |