| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tafuto, Vivian M (17)
|
MAHAC |
24.19 |
24.24 |
0.05 |
| 2 |
Buchanan, Kaley R (16)
|
MAHAC |
23.90 |
25.64 |
1.74 |
| 3 |
Hollenbach, Kaylee A (15)
|
MAHAC |
24.22 |
26.03 |
1.81 |
| 4 |
Nakano, Tomomi (15)
|
MAHAC |
25.74 |
26.25 |
0.51 |
| 5 |
Surfus, Taylor M (15)
|
MAHAC |
25.46 |
26.41 |
0.95 |
| 6 |
Hoover, Taylor C (16)
|
MACYA |
26.69 |
27.08 |
0.39 |
| 7 |
Sopko, Olivia G (15)
|
MACYA |
27.64 |
27.56 |
-0.08 |
| 8 |
Sopko, Mackenzie R (17)
|
MACYA |
27.53 |
27.83 |
0.30 |
| 9 |
Luttrell, Kiersten C (16)
|
MAHAC |
27.56 |
28.94 |
1.38 |
| 10 |
Sopko, Camryn P (15)
|
MACYA |
28.08 |
29.34 |
1.26 |
| 11 |
Ayers, Brianna M (15)
|
MAHAC |
30.42 |
29.63 |
-0.79 |
| 12 |
Alloway, Emma J (15)
|
MACYA |
32.72 |
33.72 |
1.00 |