| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Diguey, Velina (8)
|
MASSC |
|
47.17 |
- |
| 2 |
Black, Eliza (8)
|
MASSC |
|
47.88 |
- |
| 3 |
Moyer, Ava V (8)
|
MAJW |
55.05 |
48.94 |
-6.11 |
| 4 |
Gordon, Grace C (8)
|
MASSC |
54.90 |
51.56 |
-3.34 |
| 5 |
Decker, Elena R (8)
|
MAJW |
1:04.99 |
54.70 |
-10.29 |
| 6 |
Solomon, Elizabeth S (8)
|
MASSC |
58.73 |
55.37 |
-3.36 |
| 7 |
Poblador, Lianne L (8)
|
MAJW |
1:08.05 |
55.39 |
-12.66 |
| 8 |
Kirchhoff, Kathryn A (8)
|
MAJW |
1:06.25 |
56.89 |
-9.36 |
| 9 |
Dalumpines, Angel Noelle M (8)
|
MAJW |
|
58.02 |
- |
| 10 |
Bailey, Olivia A (8)
|
MAJW |
|
1:21.57 |
- |
|
Magnitsky, Anastasia S (8)
|
MAJW |
|
NS |
- |