| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Meyerhoeffer, Stella M (11)
|
MRSPAR |
35.09 |
35.45 |
0.36 |
| 2 |
Suteja, Kezia S (11)
|
MRNCAC |
38.33 |
37.96 |
-0.37 |
| 3 |
Zhu, Angela (11)
|
MRNCAC |
38.00 |
38.07 |
0.07 |
| 4 |
Alexander, Sinceraty D (11)
|
MRSPAR |
38.73 |
38.48 |
-0.25 |
| 5 |
Iannetta, Ava L (11)
|
MRSPAR |
|
38.76 |
- |
| 6 |
Weglarz, Juliet R (12)
|
MRSPAR |
41.71 |
40.07 |
-1.64 |
| 7 |
Perez, Miranda M (11)
|
MRKBM |
40.72 |
42.33 |
1.61 |
| 8 |
DiMarco, AnnaLynn (11)
|
MRSPAR |
42.53 |
43.88 |
1.35 |
| 9 |
Bauer, Demi J (11)
|
MRSPAR |
44.00 |
44.77 |
0.77 |
| 10 |
Javier, Chrisabella K (11)
|
MRAPEX |
48.12 |
46.39 |
-1.73 |
| 11 |
Royal-Smith, Jaisha (12)
|
MRKBM |
48.76 |
47.29 |
-1.47 |
| 12 |
Ramos, Samantha (12)
|
MRKBM |
50.65 |
49.04 |
-1.61 |
| 13 |
Bussey, Genesis S (12)
|
MRAPEX |
|
49.24 |
- |
| 14 |
Sharpe, Ashley B (11)
|
MRKBM |
53.50 |
50.59 |
-2.91 |
| 15 |
Leonard, Kaicey L (12)
|
MRAPEX |
58.63 |
51.46 |
-7.17 |
| 16 |
O'Mara, Brigid (11)
|
MRSPAR |
|
53.80 |
- |
| 17 |
Zhao, Joanna J (11)
|
MRAPEX |
|
53.85 |
- |
| 18 |
Vargas, Jadyn S (11)
|
MRKBM |
1:15.36 |
55.49 |
-19.87 |