| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhu, Eric Y (14)
|
MADST |
25.34 |
24.63 |
-0.71 |
| 2 |
Mislan, Trevor B (14)
|
MACBST |
25.40 |
25.30 |
-0.10 |
| 3 |
Jones, Aaron M (14)
|
MAMARA |
25.60 |
25.54 |
-0.06 |
| 4 |
Arsever, Marc L (13)
|
MAGAAC |
|
25.55 |
- |
| 5 |
Crossland, Jeffrey T (14)
|
MADST |
25.99 |
25.60 |
-0.39 |
| 6 |
Hinz, Ethan A (13)
|
MAGAAC |
27.14 |
25.71 |
-1.43 |
| 7 |
Cassidy, Tyler P (13)
|
MALMAC |
|
25.75 |
- |
| 8 |
Slupe, Collin D (13)
|
MADST |
25.72 |
25.83 |
0.11 |
| 9 |
Zheng, Lucas (13)
|
MADST |
26.83 |
26.02 |
-0.81 |
| 10 |
Benson, Jacob R (13)
|
MADST |
26.92 |
26.05 |
-0.87 |
| 11 |
Gomez Fischer, Fernando A (14)
|
MAMSA |
25.92 |
26.14 |
0.22 |
| 12 |
Patti, Alexander J (13)
|
MAGAAC |
26.11 |
26.31 |
0.20 |
| 13 |
McKenna, Luke (13)
|
MAPVAC |
27.09 |
26.39 |
-0.70 |
| 14 |
Cheng, Carlson (14)
|
MADST |
26.29 |
26.85 |
0.56 |
| 15 |
Borska, Henry (14)
|
MAMSA |
27.70 |
27.98 |
0.28 |
| 16 |
Belkadi, Ameen H (13)
|
MADST |
30.46 |
28.47 |
-1.99 |
| 17 |
Klein, William J (13)
|
MANPAC |
30.32 |
28.62 |
-1.70 |
| 18 |
Stone, Spencer (13)
|
MAPVAC |
28.88 |
28.74 |
-0.14 |
| 19 |
Mroz, Tomasz K (13)
|
MADST |
33.90 |
28.78 |
-5.12 |
| 20 |
Choi, Juneseo (13)
|
MAMSA |
|
28.85 |
- |
| 21 |
Ross, Daniel J (13)
|
MADST |
29.55 |
30.00 |
0.45 |
| 22 |
Subramanian, Vedant R (14)
|
MADST |
|
30.24 |
- |
| 23 |
Sandroni, Alec Z (13)
|
MAFSS |
32.22 |
30.37 |
-1.85 |
| 24 |
Acevedo, Nathan E (13)
|
MADST |
30.36 |
30.83 |
0.47 |
| 25 |
May, Henry M (13)
|
MAMSA |
31.40 |
31.15 |
-0.25 |
| 26 |
Clemente, Samuel M (13)
|
MAFSS |
|
33.14 |
- |
|
Davis, Andrew (14)
|
MARAD |
|
26.39 |
- |
|
Rabbiner, Jake D (13)
|
MAGAAC |
26.64 |
NS |
- |
|
Staropoli, Sam B (14)
|
MADST |
29.91 |
NS |
- |
|
Lafferty, Aaron J (14)
|
MADST |
27.35 |
NS |
- |
|
Ledwith, John C (13)
|
MARAD |
|
NS |
- |