| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Leydig, Camryn G (10)
|
MACYA |
|
2:31.45 |
- |
| 2 |
Myers, Emilee G (10)
|
MACYA |
2:59.01 |
2:35.74 |
-23.27 |
| 3 |
Surfus, Jordyn M (10)
|
MAHAC |
2:36.24 |
2:38.02 |
1.78 |
| 4 |
Kulasinsky, Katrina E (10)
|
MAHAC |
2:47.94 |
2:45.40 |
-2.54 |
| 5 |
Schmid, Romi G (10)
|
MADSC |
2:49.19 |
2:47.41 |
-1.78 |
| 6 |
Schweigert, Caroline E (9)
|
MAMARA |
3:06.89 |
3:05.20 |
-1.69 |
| 7 |
Tate, Juliana (9)
|
MACYA |
|
3:05.57 |
- |
| 8 |
Huber, Jenna C (10)
|
MADSC |
3:07.07 |
3:05.61 |
-1.46 |
| 9 |
Dye, Maggie J (9)
|
MAHAC |
3:44.79 |
3:42.44 |
-2.35 |
|
Chappell, Anna C (10)
|
MAHAC |
3:01.62 |
DQ |
- |