| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petrakis, Grace L (6)
|
MAUN6 |
53.28 |
51.93 |
-1.35 |
| 2 |
Grawl, Trina M (6)
|
MABGH |
1:00.93 |
54.61 |
-6.32 |
| 3 |
Parry, Mia J (6)
|
MALAC |
57.44 |
56.74 |
-0.70 |
| 4 |
Frank, Reese J (6)
|
NJUN4 |
59.34 |
59.13 |
-0.21 |
| 5 |
Williams, Victoria A (6)
|
MAUN@ |
1:09.24 |
1:00.46 |
-8.78 |
| 6 |
Groundwater, Margot G (6)
|
MAFSS |
1:28.44 |
1:10.20 |
-18.24 |
| 7 |
Lyons, Ella J (6)
|
MAWAC |
1:15.57 |
1:10.35 |
-5.22 |
| 8 |
Mason, Charlotte A (6)
|
MADST |
1:25.17 |
1:12.01 |
-13.16 |
| 9 |
Caraballo, Natalia R (6)
|
MAWAC |
1:32.19 |
1:12.10 |
-20.09 |
| 10 |
Blizzard, Mackenzie E (5)
|
MAOCCC |
1:19.93 |
1:13.74 |
-6.19 |
| 11 |
Koch, Claudia J (6)
|
MADST |
1:14.99 |
1:14.90 |
-0.09 |
| 12 |
Chapman, Leah H (6)
|
MADST |
1:37.05 |
1:39.04 |
1.99 |
| 13 |
Rykaczewski, Reagan E (5)
|
MADST |
1:30.22 |
1:51.13 |
20.91 |
|
Bansal, Kaylina K (6)
|
MALAC |
1:22.33 |
DQ |
- |