| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hamill, Emily (8)
|
MAMAC |
38.45 |
37.48 |
-0.97 |
| 2 |
Reasons, Kayla (7)
|
MAWAP |
41.91 |
41.75 |
-0.16 |
| 3 |
Hillier, Avery E (8)
|
MASSC |
|
42.71 |
- |
| 4 |
Shultzabarger, Lexie B (8)
|
MAFSSC |
44.03 |
43.39 |
-0.64 |
| 5 |
Tallapragada, Harshitha T (8)
|
MAMSA |
44.25 |
44.43 |
0.18 |
| 6 |
Fan, Brianna Z (8)
|
MAMSA |
47.86 |
47.01 |
-0.85 |
| 7 |
Canavan, Lillian H (8)
|
MAGRA |
48.96 |
47.04 |
-1.92 |
| 8 |
Lhulier, Ellie A (8)
|
MAWAC |
50.04 |
48.52 |
-1.52 |
| 9 |
McGlynn, Shea (8)
|
MAPFY |
47.97 |
49.92 |
1.95 |
| 10 |
Miller, Linnea R (8)
|
MAFSSC |
|
50.13 |
- |
| 11 |
Flicker, Maya R (8)
|
MAWAC |
47.92 |
50.40 |
2.48 |
| 12 |
Leary, Katherine R (8)
|
MAGRA |
|
50.62 |
- |
| 13 |
Carson, Genevieve C (8)
|
MAWAC |
58.57 |
52.59 |
-5.98 |
| 14 |
Ingram, Ainsley S (8)
|
MAGRA |
|
52.94 |
- |
| 15 |
Draper, Haley P (8)
|
MAWAC |
52.18 |
53.08 |
0.90 |
| 16 |
Heckler, Hayley H (8)
|
MAMSA |
54.86 |
55.74 |
0.88 |
| 17 |
Owens, Ava L (8)
|
MASSC |
|
55.76 |
- |
| 18 |
Horvat, Isabella E (8)
|
MAUNPA |
|
59.03 |
- |
| 19 |
Louangaphay, Kaylee (7)
|
MAACST |
1:06.15 |
1:03.69 |
-2.46 |
|
Miller, Emily E (8)
|
MAWAP |
|
54.35 |
- |