| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gravereaux, Chelsea H (13)
|
MAEMAC |
25.70 |
25.55 |
-0.15 |
| 2 |
Champagne, Katherine E (14)
|
MAEMAC |
25.12 |
25.85 |
0.73 |
| 3 |
Fahrney, Caroline F (13)
|
MAPAAC |
27.53 |
27.79 |
0.26 |
| 4 |
King, Reilly A (14)
|
MAEMAC |
27.29 |
27.98 |
0.69 |
| 5 |
Svetz, Amanda K (13)
|
MAPAAC |
27.92 |
28.19 |
0.27 |
| 6 |
Yerger, Jordyn M (14)
|
MAEMAC |
29.41 |
29.47 |
0.06 |
| 7 |
Hendricks, Michaela A (14)
|
MAPAAC |
29.19 |
29.65 |
0.46 |
| 8 |
Nelson, Caroline A (13)
|
MAEMAC |
29.08 |
29.72 |
0.64 |
| 9 |
Koller, Bridget H (14)
|
MAPAAC |
|
29.81 |
- |
| 10 |
Slauson, Taylor (14)
|
MAEAST |
30.48 |
30.89 |
0.41 |
| 11 |
Stevenson, Sophie M (14)
|
MAPAAC |
29.79 |
31.47 |
1.68 |
| 12 |
Zaffiro, Briana K (14)
|
MAPAAC |
30.93 |
31.54 |
0.61 |
| 13 |
Cincera, Isabelle O (13)
|
MAPAAC |
34.40 |
33.28 |
-1.12 |
| 14 |
Pleban, Emily R (13)
|
MAEMAC |
35.91 |
36.79 |
0.88 |
|
Champagne, Margaret M (14)
|
MAEMAC |
29.50 |
NS |
- |
|
Heier, Lena (13)
|
MAEMAC |
31.14 |
NS |
- |